Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient

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	Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient
	Lv, Huanhuan 1,3; Ouyang, Jian 1,3; Wang, Xiaoyan 1; Ma, Xiaofeng 2; Suo, Yourui 1; Wang, Honglun 1
	2015-09-14
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷号	38 期号:15 页码:1486-1493
文章类型	Article
摘要	In this study, we established a flow-rate gradient separation method to isolate four flavan-3-ol compounds with high purity from the seed coat extracts of Iris lactea by high-speed counter-current chromatography (HSCCC). First, the ethyl acetate extract was pre-separated by silica column chromatography fractionation to obtain the enriched flavan-ols sample (EFS). Then, the EFS was separated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (0.75:12.5:1:12.5, v/v/v/v) by increasing the flow rate of the mobile phase from 1.5 to 3.0mL/min after 120min. The separation temperature was 30 degrees C, the detection wavelength was 280nm and the revolution speed was 900rpm. By the optimized HSCCC method, 300mg of the EFS was separated, yielding 58mg of procyanidin B3, 72mg of procyanidin B1, 52mg of catechin, and 18mg of procyanidin B7, at a purity of 98.23%, 99.05%, 99.03%, and 96.34% in 240min. In this work, the flavan-3-ols in I. lactea are partly reviewed and the results can provide evidence to look for other rich source of procyanidins.; In this study, we established a flow-rate gradient separation method to isolate four flavan-3-ol compounds with high purity from the seed coat extracts of Iris lactea by high-speed counter-current chromatography (HSCCC). First, the ethyl acetate extract was pre-separated by silica column chromatography fractionation to obtain the enriched flavan-ols sample (EFS). Then, the EFS was separated by HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (0.75:12.5:1:12.5, v/v/v/v) by increasing the flow rate of the mobile phase from 1.5 to 3.0mL/min after 120min. The separation temperature was 30 degrees C, the detection wavelength was 280nm and the revolution speed was 900rpm. By the optimized HSCCC method, 300mg of the EFS was separated, yielding 58mg of procyanidin B3, 72mg of procyanidin B1, 52mg of catechin, and 18mg of procyanidin B7, at a purity of 98.23%, 99.05%, 99.03%, and 96.34% in 240min. In this work, the flavan-3-ols in I. lactea are partly reviewed and the results can provide evidence to look for other rich source of procyanidins.
关键词	High-speed Counter-current Chromatography Procyanidins Flow-rate Gradient Iris Lactea Pall. Var. Chinensis (Fisch.) Koidz Flavan-3-ol Preparative
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	PREPARATIVE ISOLATION ; GRAPE SEEDS ; PROANTHOCYANIDINS ; PROCYANIDINS ; BIOTRANSFORMATION ; COMPONENTS ; LEAVES
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000360437800007
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5509
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Lv, Huanhuan,Ouyang, Jian,Wang, Xiaoyan,et al. Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015,38(15):1486-1493.
APA	Lv, Huanhuan,Ouyang, Jian,Wang, Xiaoyan,Ma, Xiaofeng,Suo, Yourui,&Wang, Honglun.(2015).Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,38(15),1486-1493.
MLA	Lv, Huanhuan,et al."Separation and Purification of Four Flavan-3-ols From Iris Lactea Pall. var. Chinensis (Fisch.) Koidz by High-Speed Counter-Current Chromatography with Flow-Rate Gradient".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 38.15(2015):1486-1493.