Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway | |
| Ma, Chengjun1; Li, Gang1,2; He, Yanfeng2; Xu, Bo1,3; Mi, Xiangquan4; Wang, Honglun2; Wang, Zhenhua1 | |
| 2015-09-01 | |
| 发表期刊 | LIFE SCIENCES
 |
| 卷号 | 136页码:120-125 |
| 文章类型 | Article |
| 摘要 | Aims: Nelumbo nucifera (Gaertn.) leaves are used widely in modulating obesity in traditional Chinese medicine. Our previous work demonstrated that aporphine alkaloids from it increased the glucose consumption in mature 3T3-L1 adipocytes. However, the underlying mechanisms of this increase remain unclear. Here we investigated the modulating effects of pronuciferine and nuciferine on lipogenesis and glucose uptake in insulin resistant 3T3-L1 adipocytes in vitro. Main methods: Insulin resistant 3T3-L1 mature adipocytes were induced with dexamethasone, 3-isobutyl-methylxanthine and insulin. The lipid droplets and the intracellular triglyceride contents in mature adipocytes were detected by Oil red O staining and colorimetry respectively. The glucose uptake was measured with a fluorescent deoxyglucose analog (2-NBDG). The glucose transporter type 4 (GLUT-4) expression was measured by fluorescent-immunohistochemistry and the activation of 5'-AMP-activated protein kinase (AMPK) was detected by its alpha subunit phosphorylation. Key findings: Both nuciferine and pronuciferine treatments significantly decreased the lipid droplets and the intracellular triglyceride contents but increased the glucose uptake in the insulin resistant 3T3-L1 adipocytes. Furthermore, both pronuciferine and nuciferine showed the ability to up-regulate the expression of GLUT4, triggering the phosphorylation of AMPK in mature 3T3-L1 adipocytes, although pronuciferine exhibited a more powerful effect compared to nuciferine. Significance: In summary, all the results demonstrate that pronuciferine and nuciferine ameliorate the glucose and lipid metabolism in insulin-resistant 3T3-L1 adipocytes, which might be due to the activation of the AMPK signaling pathway. (C) 2015 Elsevier Inc. All rights reserved.; Aims: Nelumbo nucifera (Gaertn.) leaves are used widely in modulating obesity in traditional Chinese medicine. Our previous work demonstrated that aporphine alkaloids from it increased the glucose consumption in mature 3T3-L1 adipocytes. However, the underlying mechanisms of this increase remain unclear. Here we investigated the modulating effects of pronuciferine and nuciferine on lipogenesis and glucose uptake in insulin resistant 3T3-L1 adipocytes in vitro. |
| 关键词 | 3t3-l1 Adipocytes Pronuciferine Nuciferine Lipogenesis Ampk |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | INDUCED INSULIN-RESISTANCE ; NELUMBO-NUCIFERA ; PROTEIN-KINASE ; LIPID-METABOLISM ; GLUCOSE ; LEAVES ; MICE ; DIFFERENTIATION ; RESVERATROL ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| WOS类目 | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
| WOS记录号 | WOS:000361035500017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/5516 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Yantai Univ, Sch Life Sci, Ctr Mitochondria & Healthy Aging, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 3.Yantai Univ, Sch Pharm, Key Lab Mol Pharmacol & Drug Evaluat, Minist Educ China, Yantai 264005, Peoples R China 4.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Chengjun,Li, Gang,He, Yanfeng,et al. Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway[J]. LIFE SCIENCES,2015,136:120-125. |
| APA | Ma, Chengjun.,Li, Gang.,He, Yanfeng.,Xu, Bo.,Mi, Xiangquan.,...&Wang, Zhenhua.(2015).Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway.LIFE SCIENCES,136,120-125. |
| MLA | Ma, Chengjun,et al."Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway".LIFE SCIENCES 136(2015):120-125. |
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