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A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection
Li, Guoliang1,2; Tang, Chenhong3,4; Wang, Ying2; Yang, Jing2; Wu, Hongliang5; Chen, Guang2; Kong, Xiaojian2; Kong, Weiheng2; Liu, Shucheng1; You, Jinmao2,5
2015-08-01
发表期刊FOOD ANALYTICAL METHODS
卷号8期号:7页码:1804-1811
文章类型Article
摘要Semicarbazide (SEM) has been proven to extensively exist in foodstuffs due to anthropogenic factor in food processing and possesses various toxic effects on human health. Although many methods have been developed, they often require long analytical time, complex laboratory equipment, trained personnel, difficultly prepared antibodies, or relatively expensive equipment. The present study developed a new method for SEM determination by HPLC with fluorescence detection (FLD). The fluorescence reagent, 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC), was first used for SEM labeling. The fluorescent labeling conditions were optimized systematically. SEM can be labeled in only 10 min at 40 A degrees C. The labeled SEM was analyzed on an eclipse XDB-C8 column in 8 min. The new method offered the low LOD of 0.4 mu g/kg at a signal-to-noise ratio of 3 and also exhibited excellent reproducibility, precision, and accuracy. When applied to analyze several foodstuffs, it showed good applicability. The developed method has been proven to be simple, inexpensive, selective, sensitive, accurate, and reliable for SEM analysis in foodstuffs. Furthermore, this developed method should have a powerful potential in the analysis of SEM from many other food samples.; Semicarbazide (SEM) has been proven to extensively exist in foodstuffs due to anthropogenic factor in food processing and possesses various toxic effects on human health. Although many methods have been developed, they often require long analytical time, complex laboratory equipment, trained personnel, difficultly prepared antibodies, or relatively expensive equipment. The present study developed a new method for SEM determination by HPLC with fluorescence detection (FLD). The fluorescence reagent, 2-(11H-benzo[a]carbazol-11-yl) ethyl chloroformate (BCEC), was first used for SEM labeling. The fluorescent labeling conditions were optimized systematically. SEM can be labeled in only 10 min at 40 A degrees C. The labeled SEM was analyzed on an eclipse XDB-C8 column in 8 min. The new method offered the low LOD of 0.4 mu g/kg at a signal-to-noise ratio of 3 and also exhibited excellent reproducibility, precision, and accuracy. When applied to analyze several foodstuffs, it showed good applicability. The developed method has been proven to be simple, inexpensive, selective, sensitive, accurate, and reliable for SEM analysis in foodstuffs. Furthermore, this developed method should have a powerful potential in the analysis of SEM from many other food samples.
关键词Semicarbazide Hplc Fluorescence Labeling Foodstuffs
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]NITROFURAN METABOLITES ; LIQUID-CHROMATOGRAPHY ; FOOD-PRODUCTS ; AZODICARBONAMIDE ; FLOUR ; DERIVATIZATION ; AMINES ; BIUREA ; BREAD ; ASSAY
收录类别SCI
语种英语
WOS研究方向Food Science & Technology
WOS类目Food Science & Technology
WOS记录号WOS:000358326700021
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/5528
专题中国科学院西北高原生物研究所
作者单位1.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Product Proc & Safet, Zhanjiang 524000, Peoples R China
2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 27365, Peoples R China
3.Arizona State Univ, Biodesign Inst, Ctr Bioenerget, Tempe, AZ 85287 USA
4.Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA
5.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China
推荐引用方式
GB/T 7714
Li, Guoliang,Tang, Chenhong,Wang, Ying,et al. A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection[J]. FOOD ANALYTICAL METHODS,2015,8(7):1804-1811.
APA Li, Guoliang.,Tang, Chenhong.,Wang, Ying.,Yang, Jing.,Wu, Hongliang.,...&You, Jinmao.(2015).A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection.FOOD ANALYTICAL METHODS,8(7),1804-1811.
MLA Li, Guoliang,et al."A Rapid and Sensitive Method for Semicarbazide Screening in Foodstuffs by HPLC with Fluorescence Detection".FOOD ANALYTICAL METHODS 8.7(2015):1804-1811.
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