Identification and characterization of a wood-associated NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr.

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	Identification and characterization of a wood-associated NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr.
	Yang, Li 1; Hou, Yisu 1; Zhao, Xin 1; Lu, Wanxiang 2; Li, Yongli 1; Yang, Fan 1; Tang, Shaohu 1; Luo, Keming 1,3
	2015-08-01
发表期刊	TREES-STRUCTURE AND FUNCTION
卷号	29 期号:4 页码:1091-1101
文章类型	Article
摘要	Here, we isolated a NAC transcription factor PtoVNS11 from Populus tomentosa Carr. Molecular characterization showed that PtoVNS11 was involved in the regulation of secondary cell wall formation in poplar. NAC domain transcription factors are important regulators that activate the secondary wall biosynthesis in wood formation. In this study, we isolated a NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr. PtoVNS11 shares high identity with SND1 of Arabidopsis thaliana (59.6 %) and PtrWND1B (97.9 %) of P. trichocarpa. Gene expression analyses showed that PtoVNS11 gene was mainly accumulated in xylem and stem of poplar. Further, GUS expression driven by the PtoVNS11 promoter was observed in vascular tissues of vegetative and reproductive organs in transgenic Arabidopsis. Promoter deletion analysis revealed that the fragment (-283 to -171) with the secondary wall NAC-binding element was required for tissue-specific expression of the PtoVNS11 gene. Subcellular localization analysis showed that PtoVNS11:GFP fusion protein was localized in the nucleus. Transgenic poplar plants carrying 35S:PtoVNS11 exhibited dwarf phenotypes with shorter internode as compared to wild-type plants. PtoVNS11 overexpression resulted in massive deposition of lignin and increased thickening of secondary walls in transgenic poplar. Transcription analysis showed that the expression levels of many wood-associated genes were up-regulated in transgenic plants overexpressing PtoVNS11. Taken together, our results indicate that PtoVNS11 may be involved in the regulation of the secondary wall biosynthesis during wood formation in poplar.; Here, we isolated a NAC transcription factor PtoVNS11 from Populus tomentosa Carr. Molecular characterization showed that PtoVNS11 was involved in the regulation of secondary cell wall formation in poplar.
关键词	Populus Tomentosa Carr Lignin Nac Transcription Factor Secondary Cell Wall Arabidopsis Thaliana
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	SECONDARY WALL BIOSYNTHESIS ; LIGNIN BIOSYNTHESIS ; PHENYLPROPANOID METABOLISM ; COMPREHENSIVE ANALYSIS ; ARABIDOPSIS-THALIANA ; HYBRID ASPEN ; POPLAR ; GENE ; TRANSFORMATION ; REGULATORS
收录类别	SCI
语种	英语
WOS研究方向	Forestry
WOS类目	Forestry
WOS记录号	WOS:000356876600012
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5531
专题	中国科学院西北高原生物研究所
作者单位	1.Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing 400715, Peoples R China 2.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400716, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Yang, Li,Hou, Yisu,Zhao, Xin,et al. Identification and characterization of a wood-associated NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr.[J]. TREES-STRUCTURE AND FUNCTION,2015,29(4):1091-1101.
APA	Yang, Li.,Hou, Yisu.,Zhao, Xin.,Lu, Wanxiang.,Li, Yongli.,...&Luo, Keming.(2015).Identification and characterization of a wood-associated NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr..TREES-STRUCTURE AND FUNCTION,29(4),1091-1101.
MLA	Yang, Li,et al."Identification and characterization of a wood-associated NAC domain transcription factor PtoVNS11 from Populus tomentosa Carr.".TREES-STRUCTURE AND FUNCTION 29.4(2015):1091-1101.

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