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Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii
Tong, Chao1,2; Zhang, Cunfang1; Shi, Jianquan3; Qi, Hongfang3; Zhang, Renyi1,2; Tang, Yongtao1,2; Li, Guogang1,2; Feng, Chenguang1,2; Zhao, Kai1
2015-07-10
Source PublicationGENE
Volume565Issue:2Pages:201-210
SubtypeArticle
AbstractMyostatin (mstn) is an important member of TGF-beta superfamily, a muscle growth inhibitor. Though mstn has been identified in many organisms, little is known about this gene in highland fish, Gymnocypris przewalskii endemic to the Qinghai-Tibetan Plateau. In this study, we first cloned two paralogous mstn genes (mstn1 and mstn2) from G. przewalskii through homologue cloning. The 3D structures of both Mstn proteins varied in the numbers of-sheets and conformations of alpha-helices. The branch-site model showed that mstn1 has undergone positive selection, and two positively selected sites (107M and 181T) were located on the random coils of the 3D protein structure. Expression patterns indicated that the mstn1 expressed widely, while the mstn2 only expressed in the muscle and brain. During the early stage of embryo development, the expression levels of both mstn paralogous genes showed different increasing trends. These results suggest that it is diverging in two mstn paralogues of G. przewalskii via specific differences in gene structure, protein structure, selection pressure and gene expression patterns. Taken together, this study provides novel contribution on the research topics of growth related gene function and mechanism of highland fish in extreme aquatic environment on the Qinghai-Tibetan Plateau. (C) 2015 Elsevier B.V. All rights reserved.; Myostatin (mstn) is an important member of TGF-beta superfamily, a muscle growth inhibitor. Though mstn has been identified in many organisms, little is known about this gene in highland fish, Gymnocypris przewalskii endemic to the Qinghai-Tibetan Plateau. In this study, we first cloned two paralogous mstn genes (mstn1 and mstn2) from G. przewalskii through homologue cloning. The 3D structures of both Mstn proteins varied in the numbers of-sheets and conformations of alpha-helices. The branch-site model showed that mstn1 has undergone positive selection, and two positively selected sites (107M and 181T) were located on the random coils of the 3D protein structure. Expression patterns indicated that the mstn1 expressed widely, while the mstn2 only expressed in the muscle and brain. During the early stage of embryo development, the expression levels of both mstn paralogous genes showed different increasing trends. These results suggest that it is diverging in two mstn paralogues of G. przewalskii via specific differences in gene structure, protein structure, selection pressure and gene expression patterns. Taken together, this study provides novel contribution on the research topics of growth related gene function and mechanism of highland fish in extreme aquatic environment on the Qinghai-Tibetan Plateau. (C) 2015 Elsevier B.V. All rights reserved.
KeywordGymnocypris Przewalskii Myostatin Paralogue Gene Duplication Positive Selection Expression Pattern
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordDIFFERENTIAL EXPRESSION ; PHYLOGENETIC ANALYSIS ; MAXIMUM-LIKELIHOOD ; GENOME DUPLICATION ; ZEBRAFISH ; GROWTH ; EVOLUTION ; MUSCLE ; TROUT ; MUTATIONS
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000355711800007
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/5535
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Rescues Ctr Qinghai Lake Naked Carp, Xining 810016, Peoples R China
Recommended Citation
GB/T 7714
Tong, Chao,Zhang, Cunfang,Shi, Jianquan,et al. Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii[J]. GENE,2015,565(2):201-210.
APA Tong, Chao.,Zhang, Cunfang.,Shi, Jianquan.,Qi, Hongfang.,Zhang, Renyi.,...&Zhao, Kai.(2015).Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii.GENE,565(2),201-210.
MLA Tong, Chao,et al."Characterization of two paralogous myostatin genes and evidence for positive selection in Tibet fish: Gymnocypris przewalskii".GENE 565.2(2015):201-210.
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