Local adaptation of Gymnocypris przewalskii (Cyprinidae) on the Tibetan Plateau

NWIPB OpenIR

	Local adaptation of Gymnocypris przewalskii (Cyprinidae) on the Tibetan Plateau
	Zhang, Renyi 1,4; Ludwig, Arne 2; Zhang, Cunfang 1; Tong, Chao 1,4; Li, Guogang 1,4; Tang, Yongtao 1,4; Peng, Zuogang 3; Zhao, Kai 1
	2015-05-06
发表期刊	SCIENTIFIC REPORTS
卷号	5
文章类型	Article
摘要	Divergent selection among environments affects species distributions and can lead to speciation. In this article, we investigated the transcriptomes of two ecotypes of scaleless carp (Gymnocypris przewalskii przewalskii and G. p. ganzihonensis) from the Tibetan Plateau. We used a transcriptome sequencing approach to screen approximately 250,000 expressed sequence tags (ESTs) from the gill and kidney tissues of twelve individuals from the Ganzi River and Lake Qinghai to understand how this freshwater fish has adapted to an ecological niche shift from saline to freshwater. We identified 9,429 loci in the gill transcriptome and 12,034 loci in the kidney transcriptome with significant differences in their expression, of which 242 protein-coding genes exhibited strong positive selection (K-a/K-s > 1). Many of the genes are involved in ion channel functions (e.g., Ca2+-binding proteins), immune responses (e.g., nephrosin) or cellular water absorption functions (e.g., aquaporins). These results have potentially broad importance in understanding shifts from saline to freshwater habitats. Furthermore, this study provides the first transcriptome of G. przewalskii, which will facilitate future ecological genomics studies and aid in the identification of genes underlying adaptation and incipient ecological speciation.; Divergent selection among environments affects species distributions and can lead to speciation. In this article, we investigated the transcriptomes of two ecotypes of scaleless carp (Gymnocypris przewalskii przewalskii and G. p. ganzihonensis) from the Tibetan Plateau. We used a transcriptome sequencing approach to screen approximately 250,000 expressed sequence tags (ESTs) from the gill and kidney tissues of twelve individuals from the Ganzi River and Lake Qinghai to understand how this freshwater fish has adapted to an ecological niche shift from saline to freshwater. We identified 9,429 loci in the gill transcriptome and 12,034 loci in the kidney transcriptome with significant differences in their expression, of which 242 protein-coding genes exhibited strong positive selection (K-a/K-s > 1). Many of the genes are involved in ion channel functions (e.g., Ca2+-binding proteins), immune responses (e.g., nephrosin) or cellular water absorption functions (e.g., aquaporins). These results have potentially broad importance in understanding shifts from saline to freshwater habitats. Furthermore, this study provides the first transcriptome of G. przewalskii, which will facilitate future ecological genomics studies and aid in the identification of genes underlying adaptation and incipient ecological speciation.
WOS标题词	Science & Technology
关键词[WOS]	ECOLOGICAL SPECIATION ; CICHLID FISHES ; CARP NEPHROSIN ; RNA-SEQ ; TRANSCRIPTOME ; DIVERGENCE ; SELECTION ; SALINITY ; PROTEIN ; GENOMICS
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000354049500001
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5556
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Leibniz Inst Zoo & Wildlife Res, Dept Evolutionary Genet, D-10324 Berlin, Germany 3.Southwest Univ, Sch Life Sci, Key Lab Freshwater Fish Reprod & Dev, Minist Educ, Chongqing 400715, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Renyi,Ludwig, Arne,Zhang, Cunfang,et al. Local adaptation of Gymnocypris przewalskii (Cyprinidae) on the Tibetan Plateau[J]. SCIENTIFIC REPORTS,2015,5.
APA	Zhang, Renyi.,Ludwig, Arne.,Zhang, Cunfang.,Tong, Chao.,Li, Guogang.,...&Zhao, Kai.(2015).Local adaptation of Gymnocypris przewalskii (Cyprinidae) on the Tibetan Plateau.SCIENTIFIC REPORTS,5.
MLA	Zhang, Renyi,et al."Local adaptation of Gymnocypris przewalskii (Cyprinidae) on the Tibetan Plateau".SCIENTIFIC REPORTS 5(2015).