Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana | |
| Duan, Yanjiao1; Jiang, Yuanzhong1; Ye, Shenglong1; Karim, Abdul1; Ling, Zhengyi1; He, Yunqiu1; Yang, Siqi1; Luo, Keming1,2 | |
| 2015-05-01 | |
| 发表期刊 | PLANT CELL REPORTS
 |
| 卷号 | 34期号:5页码:831-841 |
| 文章类型 | Article |
| 摘要 | A salicylic acid-inducible WRKY gene, PtrWRKY73, from Populus trichocarpa , was isolated and characterized. Overexpression of PtrWRKY73 in Arabidopsis thaliana increased resistance to biotrophic pathogens but reduced resistance against necrotrophic pathogens. WRKY transcription factors are commonly involved in plant defense responses. However, limited information is available about the roles of the WRKY genes in poplar defense. In this study, we isolated a salicylic acid (SA)-inducible WRKY gene, PtrWRKY73, from Populus trichocarpa, belonging to group I family and containing two WRKY domains, a D domain and an SP cluster. PtrWRKY73 was expressed predominantly in roots, old leaves, sprouts and stems, especially in phloem and its expression was induced in response to treatment with exogenous SA. PtrWRKY73 was localized to the nucleus of plant cells and exhibited transcriptional activation. Overexpression of PtrWRKY73 in Arabidopsis thaliana resulted in increased resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae (PstDC3000), but more sensitivity to the necrotrophic fungal pathogen Botrytis cinerea. The SA-mediated defense-associated genes, such as PR1, PR2 and PAD4, were markedly up-regulated in transgenic plants overexpressing PtrWRKY73. Arabidopsis non-expressor of PR1 (NPR1) was not affected, whereas a defense-related gene PAL4 had reduced in PtrWRKY73 overexpressor plants. Together, these results indicated that PtrWRKY73 plays a positive role in plant resistance to biotrophic pathogens but a negative effect on resistance against necrotrophic pathogens.; A salicylic acid-inducible WRKY gene, PtrWRKY73, from Populus trichocarpa , was isolated and characterized. Overexpression of PtrWRKY73 in Arabidopsis thaliana increased resistance to biotrophic pathogens but reduced resistance against necrotrophic pathogens. |
| 关键词 | Populus Wrky Transcription Factor Pathogen Sa |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 关键词[WOS] | SYSTEMIC ACQUIRED-RESISTANCE ; PLANT DEFENSE RESPONSE ; DNA-BINDING PROTEINS ; BOTRYTIS-CINEREA ; GENE-EXPRESSION ; POPULUS-TRICHOCARPA ; REGULATORY ROLE ; ACTIVE OXYGEN ; FACTOR FAMILY ; CROSS-TALK |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| WOS记录号 | WOS:000352723900013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/5560 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Southwest Univ, Key Lab Ecoenvironm Gorges Reservoir Reg 3, Chongqing Key Lab Transgen Plant & Safety Control, Minist Educ,Sch Life Sci,Inst Resources Bot, Chongqing 400715, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, Northwest Inst Plateau Biol, Xining 810008, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Yanjiao,Jiang, Yuanzhong,Ye, Shenglong,et al. PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana[J]. PLANT CELL REPORTS,2015,34(5):831-841. |
| APA | Duan, Yanjiao.,Jiang, Yuanzhong.,Ye, Shenglong.,Karim, Abdul.,Ling, Zhengyi.,...&Luo, Keming.(2015).PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana.PLANT CELL REPORTS,34(5),831-841. |
| MLA | Duan, Yanjiao,et al."PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana".PLANT CELL REPORTS 34.5(2015):831-841. |
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