NWIPB OpenIR
Distribution of sialic acid receptors and experimental infections with different subtypes of influenza A viruses in Qinghai-Tibet plateau wild pika
Li, Yan1; Xiao, Haixia2; Huang, Chaobin3; Sun, Haigang4; Li, Laixing5; Su, Jingliang4; Ma, Juncai6; Liu, Di6; Wang, Han1; Liu, Wenjun1; Gao, George F.1,2,4,7,8; Li, Xiangdong3; Yan, Jinghua1
2015-04-14
Source PublicationVirology Journal
Volume12
SubtypeArticle
AbstractBackground: The plateau pika (Ochotona curzoniae) is a small rabbit-like mammal that lives at high altitudes in the Qinghai-Tibet plateau and is in close contact with birds. Following the outbreak of highly pathogenic avian influenza (HPAI) H5N1 during 2005 in the migratory birds of Qinghai Lake, two clades of H5N1 have been found in pikas. However, the influenza virus receptor distribution in different tissues of this animal and its susceptibility to influenza A viruses have remained unclear. Methods: The sialic acid receptor distribution tropism in pika was investigated using fluorescent Sambucus nigra and biotinylated Maackia amurensis I and II. Furthermore, the replication of three influenza A viruses H1N1, H3N2, and H5N1 in this animal was examined by immunohistochemistry and RT-PCR. Morphological and histopathological changes caused by infection were also analyzed with hematoxylin and eosin (H & E) staining. Results: Human influenza virus-recognizing SAa2,6Gal receptors are widely expressed in the lung, kidney, liver, spleen, duodenum, ileum, rectum, and heart, whereas avian influenza virus-recognizing SAa2,3Gal receptors are strongly expressed in the trachea and lung of pika. M1 could be detected in the lungs of pikas infected with H1N1, H3N2, and H5N1 by either immunostaining or RT-PCR, and in the brain of H5N1-infected pikas. Additionally, three subtypes of influenza A viruses were able to infect pika and caused varying degrees of pneumonia with epithelial desquamation and alveolar inflammatory cell infiltration. Slight pathological changes were observed in H1N1-infected lungs. A few small bronchi and terminal bronchioles were infiltrated by lymphocytic cells in H3N2-infected lungs. In contrast, serious lung damage, such as alveolar capillary hyperemia, edema, alveolar collapse, and lymphocytic infiltrations was observed in H5N1-infected group. Furthermore, neural system changes were present in the brains of H5N1-infected pikas. Conclusions: SAa2,6Gal receptors are extensively present in many of the tissues and organs in wild plateau pika, whereas SA2,3Gal-linked receptors are dominant on the tracheal epithelial cells. H1N1, H3N2, and H5N1 were able to infect pika and caused different degrees of pathogenic changes in the lungs. Altogether, these results suggest that wild pika has the potential to be a host for different subtypes of influenza A viruses.; Background: The plateau pika (Ochotona curzoniae) is a small rabbit-like mammal that lives at high altitudes in the Qinghai-Tibet plateau and is in close contact with birds. Following the outbreak of highly pathogenic avian influenza (HPAI) H5N1 during 2005 in the migratory birds of Qinghai Lake, two clades of H5N1 have been found in pikas. However, the influenza virus receptor distribution in different tissues of this animal and its susceptibility to influenza A viruses have remained unclear.
KeywordSialic Acid Receptors Experimental Infection Influenza a Viruses Wild Plateau Pika Pathogenic Changes
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordHUMAN AIRWAY ; H5N1 VIRUS ; OUTBREAK ; CULTURES ; TROPISM ; ILLNESS ; ORIGIN ; H17N10 ; MOUSE
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000353376200001
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/5563
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccines, Tianjin 300308, Peoples R China
3.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
4.China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China
5.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China
6.Chinese Acad Sci, Inst Microbiol, Network Informat Ctr, Beijing 100101, Peoples R China
7.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Immun & Hlth, Beijing 100101, Peoples R China
8.Chinese Ctr Dis Control & Prevent, Off Director Gen, Beijing 102206, Peoples R China
Recommended Citation
GB/T 7714
Li, Yan,Xiao, Haixia,Huang, Chaobin,et al. Distribution of sialic acid receptors and experimental infections with different subtypes of influenza A viruses in Qinghai-Tibet plateau wild pika[J]. Virology Journal,2015,12.
APA Li, Yan.,Xiao, Haixia.,Huang, Chaobin.,Sun, Haigang.,Li, Laixing.,...&Yan, Jinghua.(2015).Distribution of sialic acid receptors and experimental infections with different subtypes of influenza A viruses in Qinghai-Tibet plateau wild pika.Virology Journal,12.
MLA Li, Yan,et al."Distribution of sialic acid receptors and experimental infections with different subtypes of influenza A viruses in Qinghai-Tibet plateau wild pika".Virology Journal 12(2015).
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