Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification | |
| Chen, Xiangming1,2,3; You, Jinmao1,4; Suo, Yourui1; Fan, Baolei1,3 | |
| 2015-04-01 | |
| 发表期刊 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 卷号 | 53期号:4页码:492-497 |
| 文章类型 | Article |
| 摘要 | A new, simple and highly sensitive method for the determination of taurine, gamma-aminobutyric acid and ornithine using high-performance liquid chromatography (HPLC) with fluorescence detection is described. Three non-protein amino acids were derivatized by a novel precolumn derivatization reagent 2-[2-(dibenzocarbazol)-ethoxy]ethyl chloroformate before injected. Optimum derivatization was obtained at 40 degrees C for 5 min in the presence of sodium borate buffer (pH 9.0). Derivatives were sufficiently stable to be efficiently analyzed by HPLC without pretreatment. On a reversed-phase Hypersil BDS C8 column, the amino acids were separated in conjunction with a gradient elution with a good baseline resolution. The identification of derivatives was carried out by online postcolumn mass spectrometry with an electrospray ionization source in positive ion mode. Excellent linear responses were observed with the correlation coefficients of >0.9996, and instrument detection limits (at a signal to noise of 3 : 1) were in the range of 0.30-0.33 nmol/L. The proposed method is sensitive and reproducible for the precise determination of the amino acids from wolfberry fruit and cortex lycii samples.; A new, simple and highly sensitive method for the determination of taurine, gamma-aminobutyric acid and ornithine using high-performance liquid chromatography (HPLC) with fluorescence detection is described. Three non-protein amino acids were derivatized by a novel precolumn derivatization reagent 2-[2-(dibenzocarbazol)-ethoxy]ethyl chloroformate before injected. Optimum derivatization was obtained at 40 degrees C for 5 min in the presence of sodium borate buffer (pH 9.0). Derivatives were sufficiently stable to be efficiently analyzed by HPLC without pretreatment. On a reversed-phase Hypersil BDS C8 column, the amino acids were separated in conjunction with a gradient elution with a good baseline resolution. The identification of derivatives was carried out by online postcolumn mass spectrometry with an electrospray ionization source in positive ion mode. Excellent linear responses were observed with the correlation coefficients of >0.9996, and instrument detection limits (at a signal to noise of 3 : 1) were in the range of 0.30-0.33 nmol/L. The proposed method is sensitive and reproducible for the precise determination of the amino acids from wolfberry fruit and cortex lycii samples. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; AMINO-ACIDS ; HUMAN PLASMA ; BIOGENIC-AMINES ; REAGENT ; DERIVATIZATION ; CHLOROFORMATE ; GABA ; GLUTAMATE ; SAMPLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000356593600007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/5566 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Peoples R China 2.Binzhou Med Univ, Coll Pharm, Yantai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Xiangming,You, Jinmao,Suo, Yourui,et al. Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2015,53(4):492-497. |
| APA | Chen, Xiangming,You, Jinmao,Suo, Yourui,&Fan, Baolei.(2015).Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification.JOURNAL OF CHROMATOGRAPHIC SCIENCE,53(4),492-497. |
| MLA | Chen, Xiangming,et al."Sensitive Determination of Taurine, gamma-Aminobutyric Acid and Ornithine in Wolfberry Fruit and Cortex Lycii by HPLC with Fluorescence Detection and Online Mass Spectrometry Identification".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53.4(2015):492-497. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Chen-2015-Sensitive （279KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论