Knowledge Management System of Northwest Institute of Plateau Biology, CAS
| Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection | |
| Wu, Hongliang1,4; Li, Guoliang2; Liu, Shucheng3; Liu, Di1,4; Chen, Guang2; Hu, Na1,4; Suo, Yourui1; You, Jinmao1,2 | |
| 2015-03-25 | |
| 发表期刊 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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| 卷号 | 107页码:98-107 |
| 文章类型 | Article |
| 摘要 | A novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) follow by high-performance liquid chromatography (HPLC) with fluorescence detection. Six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by UA-DLLME using chloroform and acetone as the extraction and disperser solvents, respectively. After the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7H-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. The main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The limits of detection (LODs) and the limits of quantification (LOQs) were in the range of 0.95-1.36 ng mL(-1) and 3.17-4.55 ng mL(-1), respectively. Under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional Chinese medicinal herbs. (C) 2014 Elsevier B.V. All rights reserved.; A novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) follow by high-performance liquid chromatography (HPLC) with fluorescence detection. Six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by UA-DLLME using chloroform and acetone as the extraction and disperser solvents, respectively. After the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7H-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. The main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The limits of detection (LODs) and the limits of quantification (LOQs) were in the range of 0.95-1.36 ng mL(-1) and 3.17-4.55 ng mL(-1), respectively. Under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional Chinese medicinal herbs. (C) 2014 Elsevier B.V. All rights reserved. |
| 关键词 | Triterpenic Acids Pre-column Derivatization High-performance Liquid Chromatography Ultrasound-assisted Dispersive Liquid-liquid Microextraction Response Surface Methodology Traditional Chinese Medicinal Herbs |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| 关键词[WOS] | CAPILLARY-ZONE-ELECTROPHORESIS ; THIN-LAYER-CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; URSOLIC ACID ; OLEANOLIC ACID ; MASS-SPECTROMETRY ; LABELING REAGENT ; FATTY-ACIDS ; HPLC ; DERIVATIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Analytical ; Pharmacology & Pharmacy |
| WOS记录号 | WOS:000351116900014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/5572 |
| 专题 | 中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Hongliang,Li, Guoliang,Liu, Shucheng,et al. Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2015,107:98-107. |
| APA | Wu, Hongliang.,Li, Guoliang.,Liu, Shucheng.,Liu, Di.,Chen, Guang.,...&You, Jinmao.(2015).Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,107,98-107. |
| MLA | Wu, Hongliang,et al."Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 107(2015):98-107. |
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