Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch

NWIPB OpenIR

	Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch
	Chen, Chen 1; Chen, Tao 1; Liu, Yongling 1,2; Zou, Denglang 1,2; You, Jinmao 1; Li, Yulin 1
	2015-03-04
发表期刊	SEPARATION SCIENCE AND TECHNOLOGY
卷号	50 期号:4 页码:604-610
文章类型	Article
摘要	Gentiopicroside, mangiferin, sweroside, and isoorientin, the bioactive constituents of Swertia mussotii Franch, have various pharmacological effects, and are used in particular for treating liver disorders. However, efficient methods for their separation are not currently available. In this study, these bioactive compounds were detected in S. mussotii extracts using high-performance liquid chromatography coupled with mass spectrometry, and separated using a combination of high-speed counter-current chromatography and Sephadex LH-20 column chromatography. Their structures were determined by using H-1 and C-13 nuclear magnetic resonance spectroscopies. The results show that this method is effective for the separation and purification of bioactive compounds from S. mussotii.; Gentiopicroside, mangiferin, sweroside, and isoorientin, the bioactive constituents of Swertia mussotii Franch, have various pharmacological effects, and are used in particular for treating liver disorders. However, efficient methods for their separation are not currently available. In this study, these bioactive compounds were detected in S. mussotii extracts using high-performance liquid chromatography coupled with mass spectrometry, and separated using a combination of high-speed counter-current chromatography and Sephadex LH-20 column chromatography. Their structures were determined by using H-1 and C-13 nuclear magnetic resonance spectroscopies. The results show that this method is effective for the separation and purification of bioactive compounds from S. mussotii.
关键词	High-speed Counter-current Chromatography High Performance Liquid Chromatography Coupled With Mass Spectrometry Bioactive Compounds Swertia Mussotii Franch
WOS标题词	Science & Technology ; Physical Sciences ; Technology
关键词[WOS]	COUNTER-CURRENT CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; ISOORIENTIN ; GLYCOSIDES ; APOPTOSIS ; MICE
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Engineering
WOS类目	Chemistry, Multidisciplinary ; Engineering, Chemical
WOS记录号	WOS:000349544700015
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5573
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Chen,Chen, Tao,Liu, Yongling,et al. Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch[J]. SEPARATION SCIENCE AND TECHNOLOGY,2015,50(4):604-610.
APA	Chen, Chen,Chen, Tao,Liu, Yongling,Zou, Denglang,You, Jinmao,&Li, Yulin.(2015).Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch.SEPARATION SCIENCE AND TECHNOLOGY,50(4),604-610.
MLA	Chen, Chen,et al."Rapid Screening, Identification, Separation, and Purification of Four Bioactive Compounds from Swertia mussotii Franch".SEPARATION SCIENCE AND TECHNOLOGY 50.4(2015):604-610.