Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection

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	Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection
	Zhao Wen-Chen ; Liu Ling-Jun ; Zhao Xian-En ; Ding Chen-Xu ; Suo You-Rui ; You Jin-Mao
	2008-08-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ; Zhao WenChen,Liu LingJun,Zhao XianEn,Ding ChenXu,Suo YouRui,You JinMao.Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(8):1071-1076
摘要	On a reversed phase Hypersil BDS C-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11H-benzo [a] carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. Optimum derivatization was obtained by reacting of amino acids with BCEC-Cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. The fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. The identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. Linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids.; On a reversed phase Hypersil BDS C-18 (200 mm x 4. 6 mm, 5 mu m) column, 20 amino acids, which were derivatized using 2-(11H-benzo [a] carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) as pre-column derivatization reagent, were separated in conjunction with a gradient elution. Optimum derivatization was obtained by reacting of amino acids with BCEC-Cl at room temperature for 5 min in the presence of sodium borate catalyst in acetonitrile solvent. The fluorescence excitation and emission wavelengths were 279 nm and 380 nm respectively. The identification of amino acid derivatives from hydrolyzed bovine serum albumin and bee pollen was carried out by post-column mass spectrometry with electrospray ion source in positive ion mode. Linear correlation coefficients of the amino acid derivatives were > 0.9990, and detection limits (at signal to noise of 3:1) were 1.49 - 19.74 fmol for the labeled amino acids.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/55745
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao Wen-Chen,Liu Ling-Jun,Zhao Xian-En,et al. Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, Zhao WenChen,Liu LingJun,Zhao XianEn,Ding ChenXu,Suo YouRui,You JinMao.Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(8):1071-1076,2008.
APA	Zhao Wen-Chen,Liu Ling-Jun,Zhao Xian-En,Ding Chen-Xu,Suo You-Rui,&You Jin-Mao.(2008).Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Zhao Wen-Chen,et al."Application of 2-(11H-benzo[a] carbazol-11-yl) Ethyl Carbonochloridate as a Pro-column Derivatization Reagent of Amino Acid by High Performance Liquid Chromatography with Fluorescence Detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2008).