Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization

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	Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization
	Cai Yi-Ping 1; Sun Zhi-Wei 1; Wang Xiao-Yan 2; Suo You-Rui 2; You Jin-Mao 1,2
	2015-03-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号	43 期号:3 页码:419-423
文章类型	Article
摘要	A method for determination of 6 plant growth regulators by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) coupled with isotope-coded derivatization was developed. d(0)-10-Methyl-acridone-2-sulfonyl piperazine (d(0)-MASPz, light form) and d(3)-10-methyl-acridone-2-sulfonyl piperazine (d(3)-MASPz, heavy form) were prepared as isotope-coded derivatization reagents for carboxyl compound. The carboxylic plant growth regulator standards and real samples were derivatized by d(0)-MASPz and d(3)-MASPz, respectively. The obtained solutions were mixed at a certain ratio, and then injected for HPLC-MS/MS analysis. The light and heavy derivatives were monitored with transitions of [M + H](+)-> m/z 208.2 and [M + H](+)-> m/z 211.2, respectively. With heavy derivative as internal standard for corresponding light derivative, the global isotope internal standard quantification for 6 plant growth regulators was achieved. The results indicated that the proposed isotope-coded derivatization method could provide relative quantitative data with adequate linearity in a 10-fold dynamic range (R = 0.9991). The detection and quantitation limits were 0.19-0.34 mu M and 0.53-0.96 mu M, respectively. The relative standard deviations were <= 3.8%, and the accuracies ranged from 97. 5% to 103.8%.; A method for determination of 6 plant growth regulators by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) coupled with isotope-coded derivatization was developed. d(0)-10-Methyl-acridone-2-sulfonyl piperazine (d(0)-MASPz, light form) and d(3)-10-methyl-acridone-2-sulfonyl piperazine (d(3)-MASPz, heavy form) were prepared as isotope-coded derivatization reagents for carboxyl compound. The carboxylic plant growth regulator standards and real samples were derivatized by d(0)-MASPz and d(3)-MASPz, respectively. The obtained solutions were mixed at a certain ratio, and then injected for HPLC-MS/MS analysis. The light and heavy derivatives were monitored with transitions of [M + H](+)-> m/z 208.2 and [M + H](+)-> m/z 211.2, respectively. With heavy derivative as internal standard for corresponding light derivative, the global isotope internal standard quantification for 6 plant growth regulators was achieved. The results indicated that the proposed isotope-coded derivatization method could provide relative quantitative data with adequate linearity in a 10-fold dynamic range (R = 0.9991). The detection and quantitation limits were 0.19-0.34 mu M and 0.53-0.96 mu M, respectively. The relative standard deviations were <= 3.8%, and the accuracies ranged from 97. 5% to 103.8%.
关键词	Isotope-coded Derivatization High Performance Liquid Chromatography-tandem Mass Spectrometry Plant Growth Regulators
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	REAGENT ; SAMPLES
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000351233800004
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5575
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Cai Yi-Ping,Sun Zhi-Wei,Wang Xiao-Yan,et al. Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2015,43(3):419-423.
APA	Cai Yi-Ping,Sun Zhi-Wei,Wang Xiao-Yan,Suo You-Rui,&You Jin-Mao.(2015).Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,43(3),419-423.
MLA	Cai Yi-Ping,et al."Determination of Plant Growth Regulators in Vegetable by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Isotop-coded Derivatization".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 43.3(2015):419-423.