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A new primer-pair for sex identification of larks and wagtails
Chu, Hui1,2; Guo, Xinyi1,2; Zeng, Yan3; Zou, Xiaoyan1; Guo, Songchang1,4; Zhao, Liang1
2015-03-01
发表期刊CONSERVATION GENETICS RESOURCES
卷号7期号:1页码:19-21
文章类型Article
摘要Chromobox-helicase DNA-binding gene (CHD1) is the most reliable gene for sex identification in birds. However, the CHD1 fragments of some species are difficult to amplify. In this study, we designed a new primer set (IntP2/IntP8) that targets a conserved region of CHD1 gene. Firstly, we tested this protocol in Oriental skylark (Alauda gulgula). PCR amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. We then successfully conducted sex identification in other bird species including Tibetan lark (Melanocorypha maxima), Horned lark (Eremophila alpestris), Mongolian skylark (Melanocorypha mongolica), Asian short-toed lark (Calandrella cheleensis), Humes short-toed lark (Calandrella acutirostris), Crested lark (Galerida cristata), Yellow-headed wagtail (Motacilla citreola), White wagtail (Motacilla alba) and Ground tit (Pseudopodoces humilis Hume). Collectively, these findings support that IntP2/IntP8 primer set can be used to accurately determine sex identity in birds.; Chromobox-helicase DNA-binding gene (CHD1) is the most reliable gene for sex identification in birds. However, the CHD1 fragments of some species are difficult to amplify. In this study, we designed a new primer set (IntP2/IntP8) that targets a conserved region of CHD1 gene. Firstly, we tested this protocol in Oriental skylark (Alauda gulgula). PCR amplification produced a single band (259 bp) for males and two bands (259 and 297 bp) for females. We then successfully conducted sex identification in other bird species including Tibetan lark (Melanocorypha maxima), Horned lark (Eremophila alpestris), Mongolian skylark (Melanocorypha mongolica), Asian short-toed lark (Calandrella cheleensis), Humes short-toed lark (Calandrella acutirostris), Crested lark (Galerida cristata), Yellow-headed wagtail (Motacilla citreola), White wagtail (Motacilla alba) and Ground tit (Pseudopodoces humilis Hume). Collectively, these findings support that IntP2/IntP8 primer set can be used to accurately determine sex identity in birds.
关键词Sex Identification Chd1 Larks Wagtails
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]BIRDS
收录类别SCI
语种英语
WOS研究方向Biodiversity & Conservation ; Genetics & Heredity
WOS类目Biodiversity Conservation ; Genetics & Heredity
WOS记录号WOS:000349908000006
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/5577
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Hunan Inst Microbiol, Changsha 410009, Hunan, Peoples R China
4.Key Lab Sheep Breed & Biotechnol Xinjiang Bingtua, Shihezi 832000, Peoples R China
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GB/T 7714
Chu, Hui,Guo, Xinyi,Zeng, Yan,et al. A new primer-pair for sex identification of larks and wagtails[J]. CONSERVATION GENETICS RESOURCES,2015,7(1):19-21.
APA Chu, Hui,Guo, Xinyi,Zeng, Yan,Zou, Xiaoyan,Guo, Songchang,&Zhao, Liang.(2015).A new primer-pair for sex identification of larks and wagtails.CONSERVATION GENETICS RESOURCES,7(1),19-21.
MLA Chu, Hui,et al."A new primer-pair for sex identification of larks and wagtails".CONSERVATION GENETICS RESOURCES 7.1(2015):19-21.
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