Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)

NWIPB OpenIR

	Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)
	Zhang, Dong-Yuan ; Chen, Na ; Yang, Yan-Zhuo ; Zhang, Qian ; Liu, Jian-Quan
	2008
发表期刊	CONSERVATION GENETICS ; Zhang DongYuan,Chen Na,Yang YanZhuo, Zhang Qian,Liu JianQuan.Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae).CONSERVATION GENETICS,2008,9(2):475-477
摘要	Rhubarb is an important Traditional Chinese Medicine. However, the wild resource has been declining. In order to design appropriate conservation methods for the official species across their natural distributions, it is important to characterize their genetic diversity. Here, we describe the development of 10 new microsatellite loci for AC/TG/CCA in Rheum tanguticum. The microsatellites were enriched using the combined biotin capture method. The polymorphism of each locus was further assessed in 12 individuals from four geographically distinct populations of this species. The number of alleles ranged from three to seven and the expected heterozygosity ranged from 0.53 to 0.73. All markers have been checked in the other three species in the genus and two of them together comprise the official medicinal rhubarb resource, with R. tanguticum. These microsatellite markers could. provide a useful tool for genetic and conservation studies of the rhubarb species.; Rhubarb is an important Traditional Chinese Medicine. However, the wild resource has been declining. In order to design appropriate conservation methods for the official species across their natural distributions, it is important to characterize their genetic diversity. Here, we describe the development of 10 new microsatellite loci for AC/TG/CCA in Rheum tanguticum. The microsatellites were enriched using the combined biotin capture method. The polymorphism of each locus was further assessed in 12 individuals from four geographically distinct populations of this species. The number of alleles ranged from three to seven and the expected heterozygosity ranged from 0.53 to 0.73. All markers have been checked in the other three species in the genus and two of them together comprise the official medicinal rhubarb resource, with R. tanguticum. These microsatellite markers could. provide a useful tool for genetic and conservation studies of the rhubarb species.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/55783
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhang, Dong-Yuan,Chen, Na,Yang, Yan-Zhuo,et al. Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)[J]. CONSERVATION GENETICS, Zhang DongYuan,Chen Na,Yang YanZhuo, Zhang Qian,Liu JianQuan.Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae).CONSERVATION GENETICS,2008,9(2):475-477,2008.
APA	Zhang, Dong-Yuan,Chen, Na,Yang, Yan-Zhuo,Zhang, Qian,&Liu, Jian-Quan.(2008).Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae).CONSERVATION GENETICS.
MLA	Zhang, Dong-Yuan,et al."Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae)".CONSERVATION GENETICS (2008).