Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS

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	Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS
	Zhao, Xianen ; Wang, Honglun ; You, Jinmao ; Suo, Yourui
	2007-08-01
发表期刊	CHROMATOGRAPHIA ; Zhao Xianen,Wang Honglun,You Jinmao,Suo Yourui. Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS.CHROMATOGRAPHIA,2007,66(3-4):197-206
摘要	A method for the determination of long and short chain free fatty acids (FFAs), using 1-[2-(ptoluenesulfonate)-ethyll-2-phenylimidazole-[4,5-f-9,10-phenanthrene (TSPP) as labeling reagent, has been developed. Identification of FFA derivatives was carried out by HPLC-MS with atmospheric pressure chemical ionization (APCI) in positive ion mode. Gradient elution on an Agilent Eclipse XDB-C-8 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 200 mg of bryophyte plants and soil samples. Facile TSPP derivatization coupled with HPLC-APCI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of FFAs from biological and natural environmental samples.; A method for the determination of long and short chain free fatty acids (FFAs), using 1-[2-(ptoluenesulfonate)-ethyll-2-phenylimidazole-[4,5-f-9,10-phenanthrene (TSPP) as labeling reagent, has been developed. Identification of FFA derivatives was carried out by HPLC-MS with atmospheric pressure chemical ionization (APCI) in positive ion mode. Gradient elution on an Agilent Eclipse XDB-C-8 column gave good separation of the derivatives. Excellent linear responses were observed and good compositional data could be obtained from as little as 200 mg of bryophyte plants and soil samples. Facile TSPP derivatization coupled with HPLC-APCI-MS analysis allowed the development of a highly sensitive method for the quantitative analysis of trace level of FFAs from biological and natural environmental samples.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/55814
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhao, Xianen,Wang, Honglun,You, Jinmao,et al. Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS[J]. CHROMATOGRAPHIA, Zhao Xianen,Wang Honglun,You Jinmao,Suo Yourui. Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS.CHROMATOGRAPHIA,2007,66(3-4):197-206,2007.
APA	Zhao, Xianen,Wang, Honglun,You, Jinmao,&Suo, Yourui.(2007).Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS.CHROMATOGRAPHIA.
MLA	Zhao, Xianen,et al."Determination of free fatty acids in bryophyte plants and soil by HPLC with fluorescence detection and identification by online MS".CHROMATOGRAPHIA (2007).