Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection

NWIPB OpenIR

	Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection
	Wang, Honglun ; Ou, Lily M. L. ; Suo, Yourui ; Yu, Hua-Zhong
	2011-03-01
发表期刊	ANALYTICAL CHEMISTRY ; Wang, HL; Ou, LML; Suo, YR; Yu, HZ.Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection,ANALYTICAL CHEMISTRY,2011,83(5):1557-1563
摘要	A method for the convenient detection of lead at the parts-per-billion (ppb)-level has been developed; it uses a conventional compact disc (CD) as the platform for preparing DNAzyme assays and an unmodified optical drive of ordinary desktop/laptop computers as the readout device. In particular, by immobilization of Pb(2+) -specific DNAzyme sensing constructs on the "transparent side" of a conventional CD-R via mild surface reactions, the Pb(2+) concentration can be determined by a free diagnostic program that checks the error distribution on the CD (i.e., it extracts the number of errors in a prerecorded audio file). The reading errors increase monotonically over a wide range of Pb(2+) concentrations (from 10 nM to 1 mM), and the selectivity is confirmed by testing several other divalent cations (Zn(2+), Ba(2+), Mg(2+), Ca(2+), Cu(2+), and Hg(2+)),; A method for the convenient detection of lead at the parts-per-billion (ppb)-level has been developed; it uses a conventional compact disc (CD) as the platform for preparing DNAzyme assays and an unmodified optical drive of ordinary desktop/laptop computers as the readout device. In particular, by immobilization of Pb2+ -specific DNAzyme sensing constructs on the "transparent side" of a conventional CD-R via mild surface reactions, the Pb2+ concentration can be determined by a free diagnostic program that checks the error distribution on the CD (i.e., it extracts the number of errors in a prerecorded audio file). The reading errors increase monotonically over a wide range of Pb2+ concentrations (from 10 nM to 1 mM), and the selectivity is confirmed by testing several other divalent cations (Zn2+, Ba2+, Mg2+, Ca2+, Cu2+, and Hg2+),
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/56091
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Honglun,Ou, Lily M. L.,Suo, Yourui,et al. Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection[J]. ANALYTICAL CHEMISTRY, Wang, HL; Ou, LML; Suo, YR; Yu, HZ.Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection,ANALYTICAL CHEMISTRY,2011,83(5):1557-1563,2011.
APA	Wang, Honglun,Ou, Lily M. L.,Suo, Yourui,&Yu, Hua-Zhong.(2011).Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection.ANALYTICAL CHEMISTRY.
MLA	Wang, Honglun,et al."Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection".ANALYTICAL CHEMISTRY (2011).