Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification

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	Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification
	Fu Yanyan ; Song Cuihua ; Qin Xueqin ; Li Xiaoyan ; Ding Mingzhou ; Sun Zhiwei ; Xia Lian ; Suo Yourui ; Li Yulin ; You Jinmao
	2010-04-14
发表期刊	ACTA CHIMICA SINICA ; Fu, YY; Song, CH; Qin, XQ; Li, XY; Ding, MZ; Sun, ZW; Xia, L; Suo, YR; Li, YL; You, JM.Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification,ACTA CHIMICA SINICA,2010,68(7):653-660
摘要	On a reversed-phased Hypersil C(18) column, 10 bile acid derivatives were separated using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl 4-toluenesulfonate (BAETS) as pre-column derivatization reagent by high-performance liquid chromatography with fluorescence detection and mass spectrum identification in conjunction with a gradient elution. Optimum derivatization, giving the corresponding sensitively fluorescent derivatives, was obtained by the reacting of bile acids with BAETS at 95 degrees C for 30 min in the presence of basic catalyst potassium carbonate in dimethylsulfoxide solvent. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. The percent ionization 6 values in aqueous acetonitrile and aqueous methanol were in the range of 0%similar to 88.83% and 0%similar to 89.15% respectively. The identification of bile acid derivatives was obtained on the basis of an atmosphere-pressure chemical ionization source at positive model detection. Correlation coefficients are >0.9995, and detection limits at signal-to-noise of 3 are 0.76 similar to 1.62 ng/mL for the labeled bile acids.; On a reversed-phased Hypersil C(18) column, 10 bile acid derivatives were separated using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl 4-toluenesulfonate (BAETS) as pre-column derivatization reagent by high-performance liquid chromatography with fluorescence detection and mass spectrum identification in conjunction with a gradient elution. Optimum derivatization, giving the corresponding sensitively fluorescent derivatives, was obtained by the reacting of bile acids with BAETS at 95 degrees C for 30 min in the presence of basic catalyst potassium carbonate in dimethylsulfoxide solvent. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 272 nm/505 nm. The percent ionization 6 values in aqueous acetonitrile and aqueous methanol were in the range of 0%similar to 88.83% and 0%similar to 89.15% respectively. The identification of bile acid derivatives was obtained on the basis of an atmosphere-pressure chemical ionization source at positive model detection. Correlation coefficients are >0.9995, and detection limits at signal-to-noise of 3 are 0.76 similar to 1.62 ng/mL for the labeled bile acids.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/56161
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Fu Yanyan,Song Cuihua,Qin Xueqin,et al. Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification[J]. ACTA CHIMICA SINICA, Fu, YY; Song, CH; Qin, XQ; Li, XY; Ding, MZ; Sun, ZW; Xia, L; Suo, YR; Li, YL; You, JM.Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification,ACTA CHIMICA SINICA,2010,68(7):653-660,2010.
APA	Fu Yanyan.,Song Cuihua.,Qin Xueqin.,Li Xiaoyan.,Ding Mingzhou.,...&You Jinmao.(2010).Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification.ACTA CHIMICA SINICA.
MLA	Fu Yanyan,et al."Dual-sensitive Probe 2-(12-Oxobenzo[b]acridin-5(12H)-yl)ethyl 4-Toluenesulfonate for Bile Acids: Fluorescence Detection and APCI-MS Identification".ACTA CHIMICA SINICA (2010).