An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)

NWIPB OpenIR

	An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)
	Hu, Yanping ; Xie, Xiaolong ; Wang, Li ; Yang, Jian ; Zhang, Huaigang ; Li, Yi
	2009-06-17
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY ; Hu, YP; Xie, XL; Wang, L; Yang, J; Zhang, HG; Li, Y.An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae),AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2691-2694
摘要	Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A(260)/A(280) absorbance ratio of extracted DNA, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. The DNA preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (ISSR). This method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.; Rhubarb is an important traditional Chinese herbal drug with high secondary metabolites that interfere with DNA extraction procedures and downstream applications, such as DNA restriction and amplification. An effective and low-cost protocol for isolating genomic DNA from root of Rheum tanguticum is described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A(260)/A(280) absorbance ratio of extracted DNA, free from polysaccharide, polyphenols and tannins contaminants, ranged from 1.85 to 2.0 within the high level of purity. The DNA preparations were found suitable for analyses with restriction enzyme digestion and inter-simple sequence repeats (ISSR). This method is helpful for further studies on rhubarb at molecular level and is more cost-effective than commercial kit.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/56228
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Hu, Yanping,Xie, Xiaolong,Wang, Li,et al. An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, Hu, YP; Xie, XL; Wang, L; Yang, J; Zhang, HG; Li, Y.An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae),AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(12):2691-2694,2009.
APA	Hu, Yanping,Xie, Xiaolong,Wang, Li,Yang, Jian,Zhang, Huaigang,&Li, Yi.(2009).An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae).AFRICAN JOURNAL OF BIOTECHNOLOGY.
MLA	Hu, Yanping,et al."An effective and low-cost method for DNA extraction from herbal drugs of Rheum tanguticum (polygonaceae)".AFRICAN JOURNAL OF BIOTECHNOLOGY (2009).