LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using

NWIPB OpenIR

	LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using
	Jinmao You
	2010-10-01
发表期刊	Chromatographia ; Jinmao You.LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using,Chromatographia,2010,72(7):641-649
摘要	Abstract A pre-column derivatization method for the sensitive determination of amino acids using the tagging reagent 2-[2-(dibenzocarbazole)-ethoxy] ethyl chloroformate (DBCEC) followed by liquid chromatography with fluorescence detection has been developed. Identification of DBCEC-amino acids derivatives was by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS–MS). DBCEC can easily and quickly label amino acids, and derivatives are stable enough to be efficiently analyzed by LC. Separation of the derivatized amino acids had been optimized on Hypersil BDS C18 column. A perfect baseline separation for 20 amino acid derivatives was achieved with a ternary gradient elution program. The chromophore of dibenzocarbazole group, which comprise a large rigid planar structure with p–π conjugation system, resulted in a sensitive fluorescence detection for amino acid derivatives. The derivatized amino acids were detected with fluorescence detector with excitation maximum and emission maximum at 300 and 390 nm, respectively. Excellent linear responses were observed with coefficients of >0.9993, and detection limits were in the range of 0.78–5.13 fmol (signal-to-noise ratio of 3). The mean accuracy ranged from 83.4 to 98.7% for fluorescence detection. The mean inter-day precision for all standards was <4.2% of the expected concentration. Therefore, the proposed method was a highly sensitive and specific method for the quantitative analysis of amino acids from biological and natural environmental samples.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/56371
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jinmao You. LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using[J]. Chromatographia, Jinmao You.LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using,Chromatographia,2010,72(7):641-649,2010.
APA	Jinmao You.(2010).LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using.Chromatographia.
MLA	Jinmao You."LC-Fluorescence Detection Analysis of Amino Acids from Stellera chamaejasme L. Using".Chromatographia (2010).