Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography

doi:10.1002/jssc.201501358

NWIPB OpenIR

	Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography
	Zou, Deng-lang 1,2,3; Chen, Tao 1,2; Li, Hong-mei 1,2,3; Chen, Chen 1,2; Zhao, Jing-yang 1,2; Li, Yu-lin 1,2
	2016-04-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	39 期号:7 页码:1278-1285
文章类型	Article
摘要	This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. The ultrasound-assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high-speed counter-current chromatography without any pretreatment using n-hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by H-1 NMR spectroscopy.
关键词	Crude Extracts High-speed Counter-current Chromatography Phenolic Compounds Response Surface Methodology Terminalia Chebula Retz
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201501358
关键词[WOS]	RESPONSE-SURFACE METHODOLOGY ; POLYPHENOLIC COMPOUNDS ; PREPARATIVE ISOLATION ; ALPHA-GLUCOSIDASE ; GALLIC ACID ; PURIFICATION ; OPTIMIZATION ; ANTIOXIDANT ; FRUITS ; ULTRAFILTRATION
收录类别	SCI
语种	英语
项目资助者	General Program of Natural Science Foundation of Qinghai Province(2015-ZJ-908) ; Science and Technology Plan of Qinghai Province(2014-GX-C01 ; 2014-NS-515-2)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000374029500009
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5714
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zou, Deng-lang,Chen, Tao,Li, Hong-mei,et al. Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(7):1278-1285.
APA	Zou, Deng-lang,Chen, Tao,Li, Hong-mei,Chen, Chen,Zhao, Jing-yang,&Li, Yu-lin.(2016).Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,39(7),1278-1285.
MLA	Zou, Deng-lang,et al."Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 39.7(2016):1278-1285.