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Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC | |
Cairang, Limao ; Rui-Suo, Y. | |
2012-10-01 | |
发表期刊 | ASIAN JOURNAL OF CHEMISTRY ; Luo, JT; Hao, M; Zhang, L; Chen, JX; Zhang, LQ; Yuan, ZW; Yan, ZH; Zheng, YL; Zhang, HG; Yen, Y; Liu, DC.Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC,ASIAN JOURNAL OF CHEMISTRY,2012,24(10):4380-4384 |
摘要 | A novel, sensitive and selective HPLC-FLC method coupled with pre-column derivatization was developed for amino acid determination in Hippophae rhamnoides L. fruit from Qinghai-Tibetan plateau. The method is based on the derivatization reaction of amino group with 2[2-(dibenzocarbazole)-ethoxylethyl chloroformate (DBCEC) and the derivatives could be measured at 390 nm (excitation wavelength: 300 nm). Detection limits (calculated as the signal-to-noise ratio 3:1) were 12.85-51.68 mol for the labeled amino acids. Linearity was observed with coefficients > 0.9991 and the recoveries ranged from 92.7-105.4 %. Results in this research showed that the amount of essential amino acids accounted for 70.42 % of free amino acids and 22.68 % of the total amino acids, respectively, which benefits people's health through drinking the juice or eating the related products. Proline was dominating among free amino acids in samples, which plays a vital role for Hippophae rhamnoides L. in resisting to drought, tolerating soil salinity and lowing temperature in Qinghai-Tibetan plateau.; A novel, sensitive and selective HPLC-FLC method coupled with pre-column derivatization was developed for amino acid determination in Hippophae rhamnoides L. fruit from Qinghai-Tibetan plateau. The method is based on the derivatization reaction of amino group with 2[2-(dibenzocarbazole)-ethoxylethyl chloroformate (DBCEC) and the derivatives could be measured at 390 nm (excitation wavelength: 300 nm). Detection limits (calculated as the signal-to-noise ratio 3:1) were 12.85-51.68 mol for the labeled amino acids. Linearity was observed with coefficients > 0.9991 and the recoveries ranged from 92.7-105.4 %. Results in this research showed that the amount of essential amino acids accounted for 70.42 % of free amino acids and 22.68 % of the total amino acids, respectively, which benefits people's health through drinking the juice or eating the related products. Proline was dominating among free amino acids in samples, which plays a vital role for Hippophae rhamnoides L. in resisting to drought, tolerating soil salinity and lowing temperature in Qinghai-Tibetan plateau. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/57182 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Cairang, Limao,Rui-Suo, Y.. Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC[J]. ASIAN JOURNAL OF CHEMISTRY, Luo, JT; Hao, M; Zhang, L; Chen, JX; Zhang, LQ; Yuan, ZW; Yan, ZH; Zheng, YL; Zhang, HG; Yen, Y; Liu, DC.Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC,ASIAN JOURNAL OF CHEMISTRY,2012,24(10):4380-4384,2012. |
APA | Cairang, Limao,&Rui-Suo, Y..(2012).Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC.ASIAN JOURNAL OF CHEMISTRY. |
MLA | Cairang, Limao,et al."Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC".ASIAN JOURNAL OF CHEMISTRY (2012). |
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