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Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography
Zou, Deng-lang1,2,3; Chen, Tao1,2; Li, Hong-mei1,2,3; Chen, Chen1,2; Zhao, Jing-yang1,2; Li, Yu-lin1,2
2016-04-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
Volume39Issue:7Pages:1278-1285
SubtypeArticle
AbstractThis study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography. The ultrasound-assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high-speed counter-current chromatography without any pretreatment using n-hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by H-1 NMR spectroscopy.
KeywordCrude Extracts High-speed Counter-current Chromatography Phenolic Compounds Response Surface Methodology Terminalia Chebula Retz
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/jssc.201501358
WOS KeywordRESPONSE-SURFACE METHODOLOGY ; POLYPHENOLIC COMPOUNDS ; PREPARATIVE ISOLATION ; ALPHA-GLUCOSIDASE ; GALLIC ACID ; PURIFICATION ; OPTIMIZATION ; ANTIOXIDANT ; FRUITS ; ULTRAFILTRATION
Indexed BySCI
Language英语
Funding OrganizationGeneral Program of Natural Science Foundation of Qinghai Province(2015-ZJ-908) ; Science and Technology Plan of Qinghai Province(2014-GX-C01 ; 2014-NS-515-2)
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000374029500009
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/5724
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zou, Deng-lang,Chen, Tao,Li, Hong-mei,et al. Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(7):1278-1285.
APA Zou, Deng-lang,Chen, Tao,Li, Hong-mei,Chen, Chen,Zhao, Jing-yang,&Li, Yu-lin.(2016).Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,39(7),1278-1285.
MLA Zou, Deng-lang,et al."Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 39.7(2016):1278-1285.
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