A novel arctigenin-containing latex glove prevents latex allergy by inhibiting type I/IV allergic reactions

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	A novel arctigenin-containing latex glove prevents latex allergy by inhibiting type I/IV allergic reactions
	Wang Yong-Xin 1; Xue Dan-Ting 1; Liu Meng 1; Zhou Zheng-Min 2; Shang Jing 1,3,4
	2016-03-01
发表期刊	CHINESE JOURNAL OF NATURAL MEDICINES
卷号	14 期号:3 页码:185-195
文章类型	Article
摘要	The present study aimed at developing a natural compound with anti-allergic effect and stability under latex glove manufacturing conditions and investigating whether its anti-allergic effect is maintained after its addition into the latex. The effects of nine natural compounds on growth of the RBL-2H3 cells and mouse primary spleen lymphocytes were determined using MTT assay. The compounds included glycyrrhizin, osthole, tetrandrine, tea polyphenol, catechin, arctigenin, oleanolic acid, baicalin and oxymatrine. An ELISA assay was used for the in vitro anti-type I/IV allergy screening; in this process beta-hexosaminidase, histamine, and IL-4 released from RBL-2H3 cell lines and IFN-gamma and IL-2 released from mouse primary spleen lymphocytes were taken as screening indices. The physical stability of eight natural compounds and the dissolubility of arctigenin, selected based on the in vitro pharmacodynamaic screening and the stability evaluation, were detected by HPLC. The in vivo pharmacodynamic confirmation of arctigenin and final latex product was evaluated with a passive cutaneous anaphylaxis (PCA) model and an allergen-specific skin response model. Nine natural compounds showed minor growth inhibition on RBL-2H3 cells and mouse primary spleen lymphocytes. Baicalin and arctigenin had the best anti-type I and IV allergic effects among the natural compounds based on the in vitro pharmacodynamic screening. Arctigenin and catechin had the best physical stability under different manufacturing conditions. Arctigenin was the selected for further evaluation and proven to have anti-type I and IV allergic effects in vivo in a dose-dependent manner. The final product of the arctigenin-containing latex glove had anti-type I and IV allergic effects in vivo which were mainly attributed to arctigenin as proved from the dissolubility results. Arctigenin showed anti-type I and IV allergic effects in vitro and in vivo, with a good stability under latex glove manufacturing conditions, and a persistent anti-allergic effect after being added into the latex to prevent latex allergy.
关键词	Latex Allergy Arctigenin Rbl-2h3 Cell Mouse Primary Spleen Lymphocytes
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	NATURAL-RUBBER LATEX ; CONTACT-DERMATITIS ; MAST-CELLS ; DEGRANULATION ; CALMODULIN ; PATHWAY ; ASTHMA ; LIGNAN
收录类别	SCI
语种	英语
项目资助者	National Science and Technology Infrastructure Program of China(2012BAI30B001) ; Technological Innovation Team of Jiangsu Higher Education ; National Natural Science Foundation of China(30873156 ; New Century Excellent Talents in University(NCET-07-0851) ; Mega-projects of Science Research for the 12th Five-Year Plan of China(2011AX09401-007) ; 30672524)
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS类目	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:000373167200003
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5731
专题	中国科学院西北高原生物研究所
作者单位	1.China Pharmaceut Univ, Jiangsu Key Lab Drug Screening, Nanjing 210009, Jiangsu, Peoples R China 2.Jiangsu Guotai Int Grp Cooperat, Zhangjiagang 215600, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, Xining 810008, Peoples R China 4.China Pharmaceut Univ, Jiangsu Key Lab TCM Evaluat & Translat Res, Nanjing 210009, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Wang Yong-Xin,Xue Dan-Ting,Liu Meng,et al. A novel arctigenin-containing latex glove prevents latex allergy by inhibiting type I/IV allergic reactions[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2016,14(3):185-195.
APA	Wang Yong-Xin,Xue Dan-Ting,Liu Meng,Zhou Zheng-Min,&Shang Jing.(2016).A novel arctigenin-containing latex glove prevents latex allergy by inhibiting type I/IV allergic reactions.CHINESE JOURNAL OF NATURAL MEDICINES,14(3),185-195.
MLA	Wang Yong-Xin,et al."A novel arctigenin-containing latex glove prevents latex allergy by inhibiting type I/IV allergic reactions".CHINESE JOURNAL OF NATURAL MEDICINES 14.3(2016):185-195.