A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection

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	A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection
	Wang, Aihong ; Li, Guoliang ; You, Jinmao ; Ji, Zhongyin ; You, JM (reprint author), Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Shandong, Peoples R China.
	2013-12-01
发表期刊	JOURNAL OF SEPARATION SCIENCE ; Wang, AH; Li, GL; You, JM; Ji, ZY.A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection,JOURNAL OF SEPARATION SCIENCE,2013,36(24):3853-3859
摘要	A new fluorescent labeling reagent has been developed for the determination of fatty acids (FAs) by HPLC with fluorescence detection. The derivatization conditions including the amount of derivatization reagent, temperature, and type of catalyst were investigated, the results indicated that the reaction proceeded within 30 min at 90 degrees C in the presence of K2CO3 catalyst. The maximal yield was obtained with a four- to fivefold molar reagent excess. The derivatives exhibited strong fluorescence with an excitation maximum at (ex)= 245 nm and an emission maximum at (em)= 410 nm. Twenty-five FA derivatives were well separated by RP-HPLC on a Hypersil BDS C-8 column in combination with gradient elution. All FAs were found to give excellent linear responses with correlation coefficients >0.9992. The method gave a low LOQ of 0.85-5.5 ng/mL (S/N of 10). The developed method was employed to analyze free FAs (FFAs) composition in pomegranate samples without any purification. FFAs in samples were doubly identified by HPLC retention time and protonated molecular ion corresponding to m/z [M+H](+). This newly developed method allows a highly sensitive determination of trace FFAs from pomegranate and other foodstuffs.; A new fluorescent labeling reagent has been developed for the determination of fatty acids (FAs) by HPLC with fluorescence detection. The derivatization conditions including the amount of derivatization reagent, temperature, and type of catalyst were investigated, the results indicated that the reaction proceeded within 30 min at 90 degrees C in the presence of K2CO3 catalyst. The maximal yield was obtained with a four- to fivefold molar reagent excess. The derivatives exhibited strong fluorescence with an excitation maximum at (ex)= 245 nm and an emission maximum at (em)= 410 nm. Twenty-five FA derivatives were well separated by RP-HPLC on a Hypersil BDS C-8 column in combination with gradient elution. All FAs were found to give excellent linear responses with correlation coefficients >0.9992. The method gave a low LOQ of 0.85-5.5 ng/mL (S/N of 10). The developed method was employed to analyze free FAs (FFAs) composition in pomegranate samples without any purification. FFAs in samples were doubly identified by HPLC retention time and protonated molecular ion corresponding to m/z [M+H](+). This newly developed method allows a highly sensitive determination of trace FFAs from pomegranate and other foodstuffs.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/57385
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Aihong,Li, Guoliang,You, Jinmao,et al. A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection[J]. JOURNAL OF SEPARATION SCIENCE, Wang, AH; Li, GL; You, JM; Ji, ZY.A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection,JOURNAL OF SEPARATION SCIENCE,2013,36(24):3853-3859,2013.
APA	Wang, Aihong,Li, Guoliang,You, Jinmao,Ji, Zhongyin,&You, JM .(2013).A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection.JOURNAL OF SEPARATION SCIENCE.
MLA	Wang, Aihong,et al."A new fluorescent derivatization reagent and its application to free fatty acid analysis in pomegranate samples using HPLC with fluorescence detection".JOURNAL OF SEPARATION SCIENCE (2013).