Determination of Free Fatty Acids of Chinese Coriandrum sativum L. Using Benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate as Precolumn Labeling Reagent by LC with Fluorescence Detection

doi:10.1007/s10337-016-3071-7

NWIPB OpenIR

	Determination of Free Fatty Acids of Chinese Coriandrum sativum L. Using Benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate as Precolumn Labeling Reagent by LC with Fluorescence Detection
	Sun, Yanan 1,3; Zhang, Xia 2; Ji, Zhongyin 1,3; Song, Cuihua 2; Sun, Zhiwei 2; You, Jinmao 1,2
	2016-05-01
发表期刊	CHROMATOGRAPHIA
卷号	79 期号:9-10 页码:547-559
文章类型	Article
摘要	A new fluorescent labeling reagent, benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate (BQETS) was designed and synthesized, and it was successfully applied to the determination of fatty acids with liquid chromatography. BQETS can easily and quickly label fatty acids within 20 min at 90 A degrees C in dimethylformamide with K2CO3 as catalyst. The derivatives exhibit high stability and strong fluorescence with excitation and emission wavelengths of 247 and 401 nm, respectively. The 24 derivatives of fatty acids were completely separated by gradient elution on a Hypersil GOLD C18 column. Excellent linear responses for all fatty acids were observed with correlation coefficients of > 0.9991. The method also showed good sensitivity and precision, with limits of detection in the 0.0024-0.0206 mu g g(-1) range and relative standard deviations a parts per thousand currency sign9.6 %. This is the first time that BQETS fluorescent probe and its applications for the determination of fatty acids have been reported. Moreover, this is the first report on the comparison of free fatty acids composition in the above-ground part of Coriandrum sativum L. from different habitats in China.
关键词	Chinese Coriandrum Sativum L. Fatty Acids Lc Fluorescence
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1007/s10337-016-3071-7
关键词[WOS]	CHROMATOGRAPHY-MASS SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; GAS-CHROMATOGRAPHY ; CARBOXYLIC-ACIDS ; RISK-FACTORS ; IDENTIFICATION ; HPLC ; OMEGA-3-FATTY-ACIDS ; QUANTIFICATION ; DERIVATIZATION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21275089 ; 21475075)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000376125000003
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5754
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yanan,Zhang, Xia,Ji, Zhongyin,et al. Determination of Free Fatty Acids of Chinese Coriandrum sativum L. Using Benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate as Precolumn Labeling Reagent by LC with Fluorescence Detection[J]. CHROMATOGRAPHIA,2016,79(9-10):547-559.
APA	Sun, Yanan,Zhang, Xia,Ji, Zhongyin,Song, Cuihua,Sun, Zhiwei,&You, Jinmao.(2016).Determination of Free Fatty Acids of Chinese Coriandrum sativum L. Using Benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate as Precolumn Labeling Reagent by LC with Fluorescence Detection.CHROMATOGRAPHIA,79(9-10),547-559.
MLA	Sun, Yanan,et al."Determination of Free Fatty Acids of Chinese Coriandrum sativum L. Using Benzimidazo[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate as Precolumn Labeling Reagent by LC with Fluorescence Detection".CHROMATOGRAPHIA 79.9-10(2016):547-559.