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Cold-dependent alternative splicing of a Jumonji C domain-containing gene MtJMJC5 in Medicago truncatula
Shen, Yingfang1,2; Wu, Xiaopei1,2; Liu, Demei1; Song, Shengjing3; Liu, Dengcai1; Wang, Haiqing1
2016-05-27
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷号474期号:2页码:271-276
文章类型Article
摘要Histone methylation is an epigenetic modification mechanism that regulates gene expression in eukaryotic cells. Jumonji C domain-containing demethylases are involved in removal of methyl groups at lysine or arginine residues. The JmjC domain-only member, JMJ30/JMJD5 of Arabidopsis, is a component of the plant circadian clock. Although some plant circadian clock genes undergo alternative splicing in response to external cues, there is no evidence that JMJ30/JMJD5 is regulated by alternative splicing. In this study, the expression of an Arabidopsis JMJ30/JMJD5 ortholog in Medicago truncatula, MtJMJC5, in response to circadian clock and abiotic stresses were characterized. The results showed that MtJMJC5 oscillates with a circadian rhythm, and undergoes cold specifically induced alternative splicing. The cold induced alternative splicing could be reversed after ambient temperature returning to the normal. Sequencing results revealed four alternative splicing RNA isoforms including a full-length authentic protein encoding variant, and three premature termination condon-containing variants due to alternative 3' splice sites at the first and second intron. Under cold treatment, the variants that share a common 3' alternative splicing site at the second intron were intensively up-regulated while the authentic protein encoding variant and the premature termination condon-containing variant only undergoing a 3' alternative splicing at the first intron were down regulated. Although all the premature termination condon-harboring alternative splicing variants were sensitive to nonsense-mediated decay, the premature termination codon-harboring alternative splicing variants sharing the 3' alternative splicing site at the second intron showed less sensitivity than the one only containing the 3' alternative slicing site at the first intron under cold treatment. These results suggest that the cold-dependent alternative splicing of MtJMJC5 is likely a species or genus-specific mechanism of gene expression regulation on RNA levels, and might play a role in epigenetic regulation of the link between the circadian clock and ambient temperature fluctuation in Medicago. (C) 2016 Elsevier Inc. All rights reserved.
关键词Jmj30/jmjd5 Ortholog Jmjc Domain-containing Protein Medicago Truncatula Alternative Splicing Cold Stress
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.bbrc.2016.04.062
关键词[WOS]ARABIDOPSIS CIRCADIAN CLOCK ; NONSENSE-MEDIATED DECAY ; MESSENGER-RNA DECAY ; FLOWERING TIME ; ENVIRONMENTAL-STRESS ; EXPRESSION ; RESPONSES ; TEMPERATURE ; DEMETHYLASES ; PLANTS
收录类别SCI
语种英语
项目资助者Western Light Foundation of the Chinese Academy of Sciences ; Foundation of Application of Basic Research Project of Qinghai Province(2014-ZJ-764) ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences(KFJ-SW-STS-177)
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
WOS类目Biochemistry & Molecular Biology ; Biophysics
WOS记录号WOS:000376221700007
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/5757
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
2.Grad Univ Chinese Acad Sci, Beijing 100081, Peoples R China
3.NW Univ Xian, Coll Life Sci, Xian 710069, Peoples R China
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GB/T 7714
Shen, Yingfang,Wu, Xiaopei,Liu, Demei,et al. Cold-dependent alternative splicing of a Jumonji C domain-containing gene MtJMJC5 in Medicago truncatula[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,474(2):271-276.
APA Shen, Yingfang,Wu, Xiaopei,Liu, Demei,Song, Shengjing,Liu, Dengcai,&Wang, Haiqing.(2016).Cold-dependent alternative splicing of a Jumonji C domain-containing gene MtJMJC5 in Medicago truncatula.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,474(2),271-276.
MLA Shen, Yingfang,et al."Cold-dependent alternative splicing of a Jumonji C domain-containing gene MtJMJC5 in Medicago truncatula".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 474.2(2016):271-276.
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