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Facile and Sensitive Fluorescence Sensing of Alkaline Phosphatase Activity with Photoluminescent Carbon Dots Based on Inner Filter Effect
Li, Guoliang1,2; Fu, Huili1; Chen, Xuejie1; Gong, Peiwei1; Chen, Guang1; Xia, Lian1; Wang, Hua1; You, Jinmao1,3; Wu, Yongning2
2016-03-01
Source PublicationANALYTICAL CHEMISTRY
Volume88Issue:5Pages:2720-2726
SubtypeArticle
AbstractA simple and sensitive fluorescent assay for detecting alkaline phosphatase (ALP) based on the inner filter effect (IFE) has been proven, which is conceptually different from the previously reported ALP fluorescent assays. In this sensing platform, N-doped carbon dots (CDs) with a high quantum yield of 49% were prepared by one-pot synthesis and were directly used as a fluorophore in IFE. p-Nitrophenylphosphate (PNPP) was employed to act as an ALP substrate, and its enzyme catalytic product (p-nitrophenol (PNP)) was capable of functioning as a powerful absorber in IFE to influence the excitation of fluorophore (CDs). When in the presence of ALP, PNPP was transformed into PNP and induced the absorption band transition from 310 to 405 nm, which resulted in the complementary overlap between the absorption of PNP and the excitation of CDs. Because of the competitive absorption, the excitation of CDs was significantly weakened, resulting in the quenching of CDs. The present IFE-based sensing strategy showed a good linear relationship from 0.01 to 25 U/L (R-2 = 0.996) and provided an exciting detection limit of 0.001 U/L (signal-to-noise ratio of 3). The proposed sensing approach was successfully applied to ALP sensing in serum samples, ALP inhibitor investigation and phosphatase cell imaging. The presented IFE-based CDs fluorescence sensing strategy gives new insight on the development of the facile and sensitive optical probe for enzyme activity assay because the surface modification or the linking between the receptor and the fluorophore is no longer required.
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1021/acs.analchem.5b04193
WOS KeywordQUANTUM DOTS ; LIVING CELLS ; NANOPARTICLES ; ASSAY ; PROBE
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(31301595 ; Natural Science Foundation of Shandong Province, China(ZR2013BQ019) ; 21275089 ; 21475074)
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000371371400031
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/5765
Collection中国科学院西北高原生物研究所
Affiliation1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.China Natl Ctr Food Safety Risk Assessment, Key Labs Chem Safety & Hlth, Beijing 100050, Peoples R China
3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China
Recommended Citation
GB/T 7714
Li, Guoliang,Fu, Huili,Chen, Xuejie,et al. Facile and Sensitive Fluorescence Sensing of Alkaline Phosphatase Activity with Photoluminescent Carbon Dots Based on Inner Filter Effect[J]. ANALYTICAL CHEMISTRY,2016,88(5):2720-2726.
APA Li, Guoliang.,Fu, Huili.,Chen, Xuejie.,Gong, Peiwei.,Chen, Guang.,...&Wu, Yongning.(2016).Facile and Sensitive Fluorescence Sensing of Alkaline Phosphatase Activity with Photoluminescent Carbon Dots Based on Inner Filter Effect.ANALYTICAL CHEMISTRY,88(5),2720-2726.
MLA Li, Guoliang,et al."Facile and Sensitive Fluorescence Sensing of Alkaline Phosphatase Activity with Photoluminescent Carbon Dots Based on Inner Filter Effect".ANALYTICAL CHEMISTRY 88.5(2016):2720-2726.
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