Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry

doi:10.1016/j.chroma.2016.02.017

NWIPB OpenIR

	Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry
	Zhao, Xian-En 1,2,3,4,5,6; Lv, Tao 1,2,7; Zhu, Shuyun 1,2; Qu, Fei 1,2; Chen, Guang 1,2; He, Yongrui 1,2; Wei, Na 1,2; Li, Guoliang 1,2; Xia, Lian 1,2; Sun, Zhiwei 1,2; Zhang, Shijuan 1,2; You, Jinmao 1,2; Liu, Shu 3,4; Liu, Zhigiang 3; Sun, Jing 4,5,6; Liu, Shuying 3,4
	2016-03-11
发表期刊	JOURNAL OF CHROMATOGRAPHY A
卷号	1437 页码:49-57
文章类型	Article
摘要	This paper, for the first time, reported a speedy hyphenated technique of low toxic dual ultrasonic assisted dispersive liquid liquid microextraction (dual-UADLLME) coupled with microwave-assisted derivatization (MAD) for the simultaneous determination of 20(S)-protopanaxadiol (PPD) and 20(S)-protopanaxatriol (PPT). The developed method was based on ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection using multiple-reaction monitoring (MRM) mode. A mass spectrometry sensitizing reagent, 4'-carboxy-substituted rosamine (CSR) with high reaction activity and ionization efficiency was synthesized and firstly used as derivatization reagent. Parameters of dual-UADLLME, MAD and UHPLC-MS/MS conditions were all optimized in detail. Low toxic brominated solvents were used as extractant instead of traditional chlorinated solvents. Satisfactory linearity, recovery, repeatability, accuracy and precision, absence of matrix effect and extremely low limits of detection (LODs, 0.010 and 0.015 ng/mL for PPD and PPT, respectively) were achieved. The main advantages were rapid, sensitive and environmentally friendly, and exhibited high selectivity, accuracy and good matrix effect results. The proposed method was successfully applied to pharmacokinetics of PPD and PPT in rat plasma. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Dispersive Liquid-liquid Microextraction Microwave-assisted Derivatization Mass Spectrometry Sensitization 20(s)-protopanaxadiol 20(s)-protopanaxatriol Pharmacokinetics
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.chroma.2016.02.017
关键词[WOS]	ENVIRONMENTAL WATER SAMPLES ; SOLID-PHASE EXTRACTION ; DOG PLASMA ; RAT PLASMA ; GINSENOSIDES ; PHARMACOKINETICS ; PROTOPANAXATRIOL ; MS/MS
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(81303179 ; Natural Science Foundation of Shandong Province(ZR2013BQ018 ; Open Funds of the State Key Laboratory of Electroanalytical Chemistry(SKLEAC201506) ; Experimental Research Project of Qufu Normal University(sj201402) ; Foundation of Qufu Normal University(BSQD2012019 ; 21575007 ; ZR20138Q019 ; 2012023) ; 21405093 ; ZR20148Q009 ; 21405094 ; ZR2014BM029 ; 31301595 ; ZR2015CM040) ; 81573574 ; 21505084 ; 21475074 ; 21475075 ; 21305076)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000371556700006
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5768
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Natl Ctr Mass Spectrometry Changchun, 5625 Renmin St, Changchun 130022, Peoples R China 4.Chinese Acad Sci, Key Lab Tradit Chinese Med Chem & Mass Spectromet, Changchun Inst Appl Chem, 5625 Renmin St, Changchun 130022, Peoples R China 5.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810001, Qinghai, Peoples R China 6.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 7.Xiamen Univ, Coll Chem & Chem Engn, Dept Chem & Biochem Engn, Xiamen 361005, Fujian, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xian-En,Lv, Tao,Zhu, Shuyun,et al. Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1437:49-57.
APA	Zhao, Xian-En.,Lv, Tao.,Zhu, Shuyun.,Qu, Fei.,Chen, Guang.,...&Liu, Shuying.(2016).Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1437,49-57.
MLA	Zhao, Xian-En,et al."Dual ultrasonic-assisted dispersive liquid-liquid microextraction coupled with microwave-assisted derivatization for simultaneous determination of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol by ultra high performance liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1437(2016):49-57.