A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification

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	A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification
	Wang, Wenli ; Li, Guoliang ; Ji, Zhongyin ; Hu, Na ; You, Jinmao
	2014-08-01
发表期刊	FOOD ANALYTICAL METHODS ; Wang, WL; Li, GL; Ji, ZY; Hu, N; You, JM.A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification,FOOD ANALYTICAL METHODS,2014,7(7):1546
摘要	A stable, effective, sensitive and selective method for simultaneous determination of 11 aldehydes in foodstuffs using a novel fluorescence-labeling reagent 2-(12-benzo[b]acridin-5-(12H)-yl)-acetohydrazide (BAAH) has been developed by HPLC with fluorescence detection and mass spectrometric identification. Response surface methodology was employed to optimize the derivatization reaction between BAAH and aldehydes. The completed separation of the 11 aldehydes was achieved in as little as 18 min on a reversed-phase Hypersil BDS C-8 column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. Excellent linear coefficients were found to be of > 0.9994. This method also showed excellent reproducibility and offered the low detection limits of 0.21-0.58 nM (at a signal-to-noise ratio of 3). The developed method was successfully applied to analyze aldehydes in various foodstuffs and exhibited satisfactory applicability.; A stable, effective, sensitive and selective method for simultaneous determination of 11 aldehydes in foodstuffs using a novel fluorescence-labeling reagent 2-(12-benzo[b]acridin-5-(12H)-yl)-acetohydrazide (BAAH) has been developed by HPLC with fluorescence detection and mass spectrometric identification. Response surface methodology was employed to optimize the derivatization reaction between BAAH and aldehydes. The completed separation of the 11 aldehydes was achieved in as little as 18 min on a reversed-phase Hypersil BDS C-8 column with aqueous acetonitrile as mobile phase in conjunction with a binary gradient elution. Excellent linear coefficients were found to be of > 0.9994. This method also showed excellent reproducibility and offered the low detection limits of 0.21-0.58 nM (at a signal-to-noise ratio of 3). The developed method was successfully applied to analyze aldehydes in various foodstuffs and exhibited satisfactory applicability.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/57695
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Wenli,Li, Guoliang,Ji, Zhongyin,et al. A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification[J]. FOOD ANALYTICAL METHODS, Wang, WL; Li, GL; Ji, ZY; Hu, N; You, JM.A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification,FOOD ANALYTICAL METHODS,2014,7(7):1546,2014.
APA	Wang, Wenli,Li, Guoliang,Ji, Zhongyin,Hu, Na,&You, Jinmao.(2014).A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification.FOOD ANALYTICAL METHODS.
MLA	Wang, Wenli,et al."A novel method for trace aldehyde determination in foodstuffs based on fluorescence labeling by HPLC with fluorescence detection and mass spectrometric identification".FOOD ANALYTICAL METHODS (2014).