Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling | |
Fan, Baolei ; Chen, Guang ; Chen, Xiangming ; Suo, Yourui | |
2014 | |
发表期刊 | ASIAN JOURNAL OF CHEMISTRY
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摘要 | Rattan tea regarded as one of the most promising dietary supplements for the prevention and treatment of many chronic diseases has gained extensive attention. Free fatty acids play an important role in human body as essential nutrients. Contents of 31 free fatty acids in Rattan Tea were firstly investigated using toluene-4-sulfonic acid 2-(naphthalene-1-yl-phenyl-amino)-ethyl ester as pre-column fluorescence derivatization reagent by RE-HPLC coupled with an atmospheric chemical ionization-mass spectrometry source (APCI/ MS). The results indicated that lower values of LOD (0.52-1.53 ng mL(-1) ) provided the guarantee for trace analysis and evaluation of free fatty acids. The experimental recoveries were in the range of 88.9-103.6 %. Results of the present study indicated the highest free fatty acids content of Rattan Tea was in April. Thirteen free fatty acids were found, including C18:2 omega 6, C18:3 omega 3, omega 6 and C20:4 omega 6 essential to life. The developed method exhibited powerful potential for the systematic detection of bioactive components of medicinal plants.; Rattan tea regarded as one of the most promising dietary supplements for the prevention and treatment of many chronic diseases has gained extensive attention. Free fatty acids play an important role in human body as essential nutrients. Contents of 31 free fatty acids in Rattan Tea were firstly investigated using toluene-4-sulfonic acid 2-(naphthalene-1-yl-phenyl-amino)-ethyl ester as pre-column fluorescence derivatization reagent by RE-HPLC coupled with an atmospheric chemical ionization-mass spectrometry source (APCI/ MS). The results indicated that lower values of LOD (0.52-1.53 ng mL(-1) ) provided the guarantee for trace analysis and evaluation of free fatty acids. The experimental recoveries were in the range of 88.9-103.6 %. Results of the present study indicated the highest free fatty acids content of Rattan Tea was in April. Thirteen free fatty acids were found, including C18:2 omega 6, C18:3 omega 3, omega 6 and C20:4 omega 6 essential to life. The developed method exhibited powerful potential for the systematic detection of bioactive components of medicinal plants. |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/57774 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Fan, Baolei,Chen, Guang,Chen, Xiangming,et al. Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling[J]. ASIAN JOURNAL OF CHEMISTRY, Fan, BL; Chen, G; Chen, XM; Suo, YR.Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling,ASIAN JOURNAL OF CHEMISTRY,2014,26(1):103,2014. |
APA | Fan, Baolei,Chen, Guang,Chen, Xiangming,&Suo, Yourui.(2014).Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling.ASIAN JOURNAL OF CHEMISTRY. |
MLA | Fan, Baolei,et al."Analysis and Evaluation of Fatty Acid in Rattan Tea by Highly Selective and Sensitive HPLC-FLD-MS Method Coupled with Pre-Column Fluorescent Labeling".ASIAN JOURNAL OF CHEMISTRY (2014). |
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