The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development

doi:10.1071/RD14160

NWIPB OpenIR

	The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development
	Yang, Qi-En 1,2; Ozawa, Manabu 2,4; Zhang, Kun 2,5; Johnson, Sally E.2,3; Ealy, Alan D.2,3
	2016
发表期刊	REPRODUCTION FERTILITY AND DEVELOPMENT
卷号	28 期号:4 页码:482-490
文章类型	Article
摘要	Protein kinase C (PKC) delta (PRKCD) is a member of the novel PKC subfamily that regulates gene expression in bovine trophoblast cells. Additional functions for PRKCD in early embryonic development in cattle have not been fully explored. The objectives of this study were to describe the expression profile of PRKCD mRNA in bovine embryos and to examine its biological roles during bovine embryo development. Both PRKCD mRNA and protein are present throughout early embryo development and increases in mRNA abundance are evident at morula and blastocyst stages. Phosphorylation patterns are consistent with detection of enzymatically active PRKCD in bovine embryos. Exposure to a pharmacological inhibitor (rottlerin) during early embryonic development prevented development beyond the eight- to 16-cell stage. Treatment at or after the 16-cell stage reduced blastocyst development rates, total blastomere numbers and inner cell mass-to-trophoblast cell ratio. Exposure to the inhibitor also decreased basal interferon tau (IFNT) transcript abundance and abolished fibroblast growth factor-2 induction of IFNT expression. Furthermore, trophoblast adhesion and proliferation was compromised in hatched blastocysts. These observations provide novel insights into PRKCD mRNA expression profiles in bovine embryos and provide evidence for PRKCD-dependent regulation of embryonic development, gene expression and post-hatching events.
关键词	Embryonic Development Embryonic Gene Expression Post-hatching Development
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1071/RD14160
关键词[WOS]	INTERFERON-TAU EXPRESSION ; FIBROBLAST GROWTH-FACTORS ; IN-VITRO ; CONCEPTUS DEVELOPMENT ; BLASTOCYST FORMATION ; MOUSE EMBRYOS ; PKC ISOFORMS ; COMPACTION ; STAGE ; TROPHECTODERM
收录类别	SCI
语种	英语
项目资助者	National Research Initiative Competitive Grant from the USA Department of Agriculture's National Institute of Food and Agriculture(2008-35203-19106)
WOS研究方向	Developmental Biology ; Reproductive Biology ; Zoology
WOS类目	Developmental Biology ; Reproductive Biology ; Zoology
WOS记录号	WOS:000370856400010
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5777
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adapt & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 2.Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA 3.Virginia Polytech Inst & State Univ, Dept Anim & Poultry Sci, 175 W Campus Dr, Blacksburg, VA 24061 USA 4.Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan 5.Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA
推荐引用方式 GB/T 7714	Yang, Qi-En,Ozawa, Manabu,Zhang, Kun,et al. The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development[J]. REPRODUCTION FERTILITY AND DEVELOPMENT,2016,28(4):482-490.
APA	Yang, Qi-En,Ozawa, Manabu,Zhang, Kun,Johnson, Sally E.,&Ealy, Alan D..(2016).The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development.REPRODUCTION FERTILITY AND DEVELOPMENT,28(4),482-490.
MLA	Yang, Qi-En,et al."The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development".REPRODUCTION FERTILITY AND DEVELOPMENT 28.4(2016):482-490.