Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim

doi:10.1016/j.ijbiomac.2015.10.038

NWIPB OpenIR

	Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim
	Han, Lijuan 1,2; Suo, Yourui 1,3,4; Yang, Yongjing 1,2; Meng, Jing 1,2; Hu, Na 1,2
	2016-04-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷号	85 页码:655-666
文章类型	Article
摘要	In this study, the extraction of water-soluble polysaccharides (BDPs) from Berberis dasystachya Maxim using dynamic microwave-assisted extraction (DMAE) was discussed. A Box-Behnken design combined with response surface methodology has been employed to optimize extraction parameters of DMAE. The BDPs have been analyzed in order to identify a variety of chemical properties. Antioxidant and anti-tumor activities in vitro have been studied by DPPH, ABTS, reducing power assay, and MTT assay, respectively. The results obtained showed that the optimal extraction conditions were as follows: ratio of water to raw material (X-1) 25.84 mg/L, extraction power (X-2) 433.13 W, extraction time (X-3) 35.18 min, and the maximum yield of extraction was 6.472 +/- 0.384%, which was in good agreement with the predicted value. The physicochemical tests demonstrated that the BDPs mainly consist of rhamnose, arabinose, xylose, mannose, glucose and lactose in a molar ratio of 1:17.3:1.33:7:2.33:1.78; the average molecular weight of the BDPs was estimated to be from 2.95 x 10(5) and 1.52 x 10(3) Da, respectively. Furthermore, the BDPs exhibited effective antioxidant and anti-proliferative properties in vitro. Such pharmaceutical activities could prove useful for potential future applications involving the berries of B. dasystachya Maxim. (C) 2015 Elsevier B.V. All rights reserved.
关键词	B. Dasystachya Maxim Polysaccharides Response Surface Methodology Antioxidant And Anti-proliferative
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.ijbiomac.2015.10.038
关键词[WOS]	MICROWAVE-ASSISTED EXTRACTION ; RESPONSE-SURFACE METHODOLOGY ; WATER-SOLUBLE POLYSACCHARIDE ; ANTIOXIDANT ACTIVITIES ; STRUCTURAL-CHARACTERIZATION ; ANTICANCER ACTIVITY ; THERMAL-STABILITY ; LYCIUM-RUTHENICUM ; CHEMICAL-ANALYSIS ; IMMUNOMODULATION
收录类别	SCI
语种	英语
项目资助者	Foundation of International S&T Cooperation Program of China(S2014GR0058)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:000370886800075
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/5785
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qinghai Univ, Xining 810001, Qinghai, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Han, Lijuan,Suo, Yourui,Yang, Yongjing,et al. Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,85:655-666.
APA	Han, Lijuan,Suo, Yourui,Yang, Yongjing,Meng, Jing,&Hu, Na.(2016).Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,85,655-666.
MLA	Han, Lijuan,et al."Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 85(2016):655-666.