Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates

NWIPB OpenIR

	Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates
	Pei, Jinjin ; Jiang, Hai ; Li, Xinsheng ; Jin, Wengang ; Tao, Yanduo
	2017-12-06
发表期刊	AMB EXPRESS
摘要	Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of magnetic liposome adsorption combined with reversed-phase high performance liquid chromatography (RP-HPLC) was developed to screen for and purify the antimicrobial peptides. Two antimicrobial peptides were obtained and their amino acid sequences were determined by N-sequencing, namely Arg-Val-Met-Phe-Lys-Trp-Ala and Lys-Val-Ile-Ser-Met-Ile. The antimicrobial activity spectra of Arg-Val-Met-Phe-Lys-Trp-Ala included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua, Escherichia coli, Enterobacter cloacae and Salmonella paratyphi, while the Lys-Val-Ile-Ser-Met-Ile peptide shows not only bacterial growth inhibition but also of fungi. Haemolytic testing suggested that these two antimicrobial peptides could be considered to have no haemolytic effect at their minimum inhibitory concentrations (MICs).
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59392
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Pei, Jinjin,Jiang, Hai,Li, Xinsheng,et al. Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates[J]. AMB EXPRESS,2017.
APA	Pei, Jinjin,Jiang, Hai,Li, Xinsheng,Jin, Wengang,&Tao, Yanduo.(2017).Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates.AMB EXPRESS.
MLA	Pei, Jinjin,et al."Antimicrobial peptides sourced from post-butter processing waste yak milk protein hydrolysates".AMB EXPRESS (2017).