A highly sensitive turn-on fluorescent chemosensor for recognition of Zn(II) ions and its application in live cells imaging

NWIPB OpenIR

	A highly sensitive turn-on fluorescent chemosensor for recognition of Zn(II) ions and its application in live cells imaging
	Wang, Peng; Wu, Jiang
	2020
发表期刊	JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
卷号	386
摘要	A novel and simple fluorescent chemosensor, DH, was synthesized. As designed, DH displayed excellent selectivity exhibiting a "turn-on" response for Zn2+ over other relevant metal ions based on PET mechanism in 100% aqueous media, and the binding constant of Zn2+ binding to DH was calculated to be 7.27 x 10(8) M-2. Importantly, DH exhibited high sensitivity as a Zn2+ fluorescent chemosensor with a limit of detection (LOD) of 19.25 nM, which is lower than WHO guidelines (76.0 mu M). Moreover, the 2:1 stoichiometry between the chemosensor DH and Zn2+ was verified by Job's plot, fluorescent titration and high resolution mass spectroscopy. In addition, the recognition process of DH toward Zn2+ was chemically reversible and recyclable by adding Na(2)EDTA. Moreover, due to its good biocompatibility and low cytotoxicity, DH was successfully applied to the fluorescence imaging of Zn2+ and Na(2)EDTA in living HK2 cells.
关键词	Histidine Chemosensor Reversibility Zn(II) ions Cells imaging
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59669
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Peng,Wu, Jiang. A highly sensitive turn-on fluorescent chemosensor for recognition of Zn(II) ions and its application in live cells imaging[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,2020,386.
APA	Wang, Peng,&Wu, Jiang.(2020).A highly sensitive turn-on fluorescent chemosensor for recognition of Zn(II) ions and its application in live cells imaging.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,386.
MLA	Wang, Peng,et al."A highly sensitive turn-on fluorescent chemosensor for recognition of Zn(II) ions and its application in live cells imaging".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY 386(2020).