Simultaneous identification and quantification of five flavonoids in the seeds of Rheum palmatum L. by using accelerated solvent extraction and HPLC-PDA- ESI/MSn

NWIPB OpenIR

	Simultaneous identification and quantification of five flavonoids in the seeds of Rheum palmatum L. by using accelerated solvent extraction and HPLC-PDA- ESI/MSn
	Tan, Liang; Ji, Tian; Jiang, Guijuan; Hu, Fengzu
	2019
发表期刊	ARABIAN JOURNAL OF CHEMISTRY
卷号	12 期号:7
摘要	In this study, flavonoids were extracted using accelerated solvent extraction (ASE) with 80% aqueous methanol. A high-performance liquid chromatography equipped with photodiode array detection and coupled with an electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI/MSn) method has been developed for rapid identification and quantification of flavonoids in the plant extract of Rheum palmatum L. (RPL) seeds from Qinghai. Ten flavonoids from methanolic extract of RPL seeds were screened, of which epicatechin, myricetin, hyperoside, quercitrin and quercetin were identified and quantitatively analyzed for the first time. The absorbance was monitored at 280 nm for epicatechin and 360 nm for other four flavonoids. It was found that the calibration curves for all five analytes showed a good linearity (r(2) > 0.999) within the test range and the data of the limit of detection (LOD) and limit of quantification (LOQ) for all investigated compounds were less than 2.98 mu g mL(-1) based on PDA. The relative standard deviation (R.S.D) values of the intra- and inter-day precisions were 0.98% and 1.65%, respectively. The range of mean recoveries of the five components was 91.3-93.9%, and the R.S.D was 1.3-2.9%. It was also found that the content of quercitrin is the highest in RPL seeds while the contents of epicatechin and myricetin are relatively lower. In addition, the validation procedure confirmed that this method was suitable for providing quality control evaluation of RPL seeds to ensure the therapeutic benefits. (C) 2014 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.
关键词	Seeds of Rheum palmatum L. Identification Quantification Flavonoids Accelerated solvent extraction HPLC-PDA-ESI/MSn
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59680
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Tan, Liang,Ji, Tian,Jiang, Guijuan,et al. Simultaneous identification and quantification of five flavonoids in the seeds of Rheum palmatum L. by using accelerated solvent extraction and HPLC-PDA- ESI/MSn[J]. ARABIAN JOURNAL OF CHEMISTRY,2019,12(7).
APA	Tan, Liang,Ji, Tian,Jiang, Guijuan,&Hu, Fengzu.(2019).Simultaneous identification and quantification of five flavonoids in the seeds of Rheum palmatum L. by using accelerated solvent extraction and HPLC-PDA- ESI/MSn.ARABIAN JOURNAL OF CHEMISTRY,12(7).
MLA	Tan, Liang,et al."Simultaneous identification and quantification of five flavonoids in the seeds of Rheum palmatum L. by using accelerated solvent extraction and HPLC-PDA- ESI/MSn".ARABIAN JOURNAL OF CHEMISTRY 12.7(2019).