NWIPB OpenIR
beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition
Cui, Yulei; Jiang, Lei; Yu, Ruitao; Shao, Yun; Mei, Lijuan; Tao, Yanduo
2019
发表期刊JOURNAL OF ETHNOPHARMACOLOGY
卷号243
摘要Ethnopharmacological relevance: The plant Arenaria kansuensis is used in traditional medicine to treat lung inflammation for a long time. However, the anti-pulmonary fibrosis effect and its corresponding bioactive constituents of this plant have not been studied extensively. Aim of the study: The purpose of this study was to investigate the anti-pulmonary fibrosis effect and its corresponding bioactive constituents of A. kansuensis and its possible mechanism. Materials and methods: In vivo experiment, the anti-pulmonary fibrosis effects of the fraction (Part1) enriched from ethyl acetate extracts of the whole plant A. kansuensis were evaluated through bleomycin (BLM)-induced pulmonary fibrosis mice (five groups, n = 10) daily at doses of 50, 100 and 150 mg/kg for 15 days. In vitro experiment, the anti-inflammation and reversed epithelial-mesenchymal transition (EMT) effect of 12 beta-carboline alkaloids isolated from Part1 were evaluated through lipopolysaccharide (LPS)-induced RAW264.7 inflammatory cell model and TGF-beta 1 induced A549 cell model. Results: In this study, a fraction named Part1 extracted from Arenaria kansuensis presented strong anti-pulmonary fibrosis effect at the dose of 150 mg/kg. Vivo experiments showed that the survival rate and body weight of mice significantly increased after Partl treatment. Part1 could significantly inhibit the initial of inflammation, deposition of collagen and expression of TGF-beta 1 and alpha-SMA, moreover, the expression of E-cadherin was significantly elevated after administration of Part1. All the cure effects of Part1 were in dose dependent manner. A total of 12 beta-carboline alkaloids were identified in Part1 and they all showed suppressive effect on inflammatory cytokines including MCP-1, TNF-alpha, IL-6 and IL-1 beta through inhibition of NF-kb/p65 phosphorylation, and that epithelial-mesenchymal transition (EMT) process was reversed by different compounds in different levels. The expression of indicators of EMT including alpha-SMA, vimentin and E-cadherin was significantly improved after given different beta-carboline alkaloids. Conclusions: This study showed that antifibrogenic effect of beta-carboline alkaloids was due to inhibiting the initial of inflammation through NF-kb/p65 pathway and reversing the process of EMT.
关键词beta-carboline alkaloids Pulmonary fibrosis NF-kb/p65 EMT Arenaria kansuensis
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/59683
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Cui, Yulei,Jiang, Lei,Yu, Ruitao,et al. beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,243.
APA Cui, Yulei,Jiang, Lei,Yu, Ruitao,Shao, Yun,Mei, Lijuan,&Tao, Yanduo.(2019).beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition.JOURNAL OF ETHNOPHARMACOLOGY,243.
MLA Cui, Yulei,et al."beta-carboline alkaloids attenuate bleomycin induced pulmonary fibrosis in mice through inhibiting NF-kb/p65 phosphorylation and epithelial-mesenchymal transition".JOURNAL OF ETHNOPHARMACOLOGY 243(2019).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cui, Yulei]的文章
[Jiang, Lei]的文章
[Yu, Ruitao]的文章
百度学术
百度学术中相似的文章
[Cui, Yulei]的文章
[Jiang, Lei]的文章
[Yu, Ruitao]的文章
必应学术
必应学术中相似的文章
[Cui, Yulei]的文章
[Jiang, Lei]的文章
[Yu, Ruitao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。