Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle-pressure chromatogram isolated gel column coupled with two-dimensional liquid chromatography

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	Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle-pressure chromatogram isolated gel column coupled with two-dimensional liquid chromatography
	Pan, Guoqing; Shen, Jianwei; Ma, Yuhua; He, Yanfeng; Bao, Yi; Li, Rongrong; Wang, Shunshan; Wang, Qi; Lin, Pengcheng; Dang, Jun
	2019
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	42 期号:20
摘要	We established a two-dimensional strong cation exchange/reversed-phase liquid chromatography protocol to isolate and purify isoquinoline alkaloids from Corydalis impatiens. Isoquinoline alkaloids were first enriched from a C. impatiens extract in which liposoluble components were removed using a medium-pressure chromatographic tower containing middle chromatogram isolated gel. A strong cation exchange column was employed to separate and obtain 30 fractions. We chose fractions 22-29 for reversed-phase liquid chromatography purification using characteristic isoquinoline alkaloid ultraviolet absorption spectra. Several isoquinoline alkaloid fractions (22-29) were further separated, and those of low resolution were isolated via two-dimensional liquid chromatography in the orthogonal plane. A total of eight novel isoquinoline alkaloids with characteristic ultraviolet spectra were obtained from C. impatiens. We thus demonstrate the benefits of off-line two-dimensional strong cation exchange/reversed-phase liquid chromatography to isolate isoquinoline alkaloids from C. impatiens.
关键词	Corydalis impatiens isoquinoline alkaloids preparative separation two-dimensional liquid chromatography
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59689
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Pan, Guoqing,Shen, Jianwei,Ma, Yuhua,et al. Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle-pressure chromatogram isolated gel column coupled with two-dimensional liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(20).
APA	Pan, Guoqing.,Shen, Jianwei.,Ma, Yuhua.,He, Yanfeng.,Bao, Yi.,...&Dang, Jun.(2019).Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle-pressure chromatogram isolated gel column coupled with two-dimensional liquid chromatography.JOURNAL OF SEPARATION SCIENCE,42(20).
MLA	Pan, Guoqing,et al."Preparative separation of isoquinoline alkaloids from Corydalis impatiens using a middle-pressure chromatogram isolated gel column coupled with two-dimensional liquid chromatography".JOURNAL OF SEPARATION SCIENCE 42.20(2019).