Combined chromatographic strategy based on macroporous resin, high-speed counter-current chromatography and preparative HPLC for systematic separation of seven antioxidants from the fruit of Terminalia billerica

NWIPB OpenIR

	Combined chromatographic strategy based on macroporous resin, high-speed counter-current chromatography and preparative HPLC for systematic separation of seven antioxidants from the fruit of Terminalia billerica
	Yang, Xue; Shen, Cheng; Li, Hongmei; Wang, Nana; Ma, Jialin; Wang, Shuo; Zhao, Jingyang; Chen, Jilin; Yang, Li; Chen, Tao; Li, Yulin
	2019
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	42 期号:20
摘要	In the present study, combined chromatographic strategy based on macroporous resin, high-speed counter-current chromatography and preparative high-performance liquid chromatography for systematic separation of antioxidants from crude samples guided by high-performance liquid chromatography with 1,1-diphenyl-2-picrylhydrazyl has been successfully established. Based on this strategy, seven antioxidants including isorugosin A, beta-1,2,3,6-tetragalloyl-D-glucose, chebulinic acid, 1,2,3,4,6-penta-Ogalloyl-beta-D-glucose, chebulagic acid, ethyl gallate, and gallic acid were obtained from the fruit of Terminalia billerica. First, high-performance liquid chromatography with 1,1-diphenyl-2-picrylhydrazyl experiment showed the presence of seven main antioxidants in the crude extract of the fruit of Terminalia billerica. Then, a macroporous resin column chromatography method was developed for the enrichment of these seven antioxidants. Finally, an efficient method based on high-speed counter-current chromatography and preparative high-performance liquid chromatography was developed for the separation of these antioxidants. In the selection of solvent systems, it was found that acetic acid could be a good regulator for modifying the partition coefficient values of tannins. The present study provides a reference for systematic separation of antioxidants from crude samples. Considering the general existence of antioxidants in crude samples, this combined chromatographic strategy might lead to broader application prospects.
关键词	antioxidants macroporous resin systematic separation Terminalia bellirica traditional Chinese medicine
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59690
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Xue,Shen, Cheng,Li, Hongmei,et al. Combined chromatographic strategy based on macroporous resin, high-speed counter-current chromatography and preparative HPLC for systematic separation of seven antioxidants from the fruit of Terminalia billerica[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(20).
APA	Yang, Xue.,Shen, Cheng.,Li, Hongmei.,Wang, Nana.,Ma, Jialin.,...&Li, Yulin.(2019).Combined chromatographic strategy based on macroporous resin, high-speed counter-current chromatography and preparative HPLC for systematic separation of seven antioxidants from the fruit of Terminalia billerica.JOURNAL OF SEPARATION SCIENCE,42(20).
MLA	Yang, Xue,et al."Combined chromatographic strategy based on macroporous resin, high-speed counter-current chromatography and preparative HPLC for systematic separation of seven antioxidants from the fruit of Terminalia billerica".JOURNAL OF SEPARATION SCIENCE 42.20(2019).