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Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification
Su, Yiyun; Wu, Di; Chen, Jian; Chen, Guang; Hu, Na; Wang, Honglun; Wang, Panxue; Han, Haoyu; Li, Guoliang; Wut, Yongning
2019
发表期刊ANALYTICAL CHEMISTRY
卷号91期号:18
摘要The exploration of nanomaterials with mimic enzyme activity (named nanozyme) has gained extensive attention in the fields of advanced analytical chemistry and materials science. Herein, the gold nanoparticles doped covalent organic frameworks (COFs) were prepared, which exhibited not only excellent mimic nitroreductase activity but also robust stability. By replacing the traditional natural enzyme tag in an enzyme-linked immunosorbent assay (ELISA), we employed the proposed nanozyme to label the detecting antibody. According to the catalytic properties of the nanozyme, 4-nitrothiophenol (4-NTP) was introduced as the substrate, which can be transformed to 4-aminothiophenol (4-ATP) in the presence of NaBH4. In a surface enhanced Raman scattering (SERS) assay, 4-ATP was capable of functioning as a powerful bridge to connect the gold nanostars (with excellent SERS performance) by both the Au-S bond and electrostatic force to further produce a Raman "hot spot". Meanwhile, the Raman signal of 4-nitrothiophenol at 1573 cm(-1) was weakened, and a new signal at 1591 cm(-1) generated by 4-ATP was turned on, leading to the generation of a ratiometric SERS signal. Based on this performance, a ratiometric nanozyme-linked immunosorbent assay (NELISA) strategy was developed delicately, which was applied to detect beta-lactoglobulin (allergenic protein) by monitoring the ratiometric signal of I-1591/I-1573 with a limit of detection (LOD) of 0.01 ng/mL. The linear range is 25.65-6.2 x 10(4) ng/mL, covering more than 3 orders of magnitude. The developed method showed many advantages such as low-cost, higher recovery, and lower cross-reactivity, providing new insight into the application of SERS technology for trace target analysis.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/59699
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Su, Yiyun,Wu, Di,Chen, Jian,et al. Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification[J]. ANALYTICAL CHEMISTRY,2019,91(18).
APA Su, Yiyun.,Wu, Di.,Chen, Jian.,Chen, Guang.,Hu, Na.,...&Wut, Yongning.(2019).Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification.ANALYTICAL CHEMISTRY,91(18).
MLA Su, Yiyun,et al."Ratiometric Surface Enhanced Raman Scattering Immunosorbent Assay of Allergenic Proteins via Covalent Organic Framework Composite Material Based Nanozyme Tag Triggered Raman Signal "Turn-on" and Amplification".ANALYTICAL CHEMISTRY 91.18(2019).
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