IFN-gamma inhibits 5-HT-induced melanin biosynthesis via downregulation of 5-HT receptors in vivo/in vitro

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	IFN-gamma inhibits 5-HT-induced melanin biosynthesis via downregulation of 5-HT receptors in vivo/in vitro
	Cai, Minxuan; Zhou, Liangliang; Liao, Jun; Huang, Qiaoling; Xia, Zhenjiang; Shang, Jing
	2019
发表期刊	JOURNAL OF PHARMACOLOGICAL SCIENCES
卷号	141 期号:1
摘要	Hypopigmentation disorders, such as vitiligo, are difficult for treatment due to complicated pathogenesis, resulting from multiple factors including neural and immune elements. 5-HT and IFN-gamma both play crucial roles in these skin diseases. However, the interactions between 5-HT and IFN-gamma in regulation of melanogenesis is still unknown. Our study aimed at exploring whether IFN-gamma affects 5-HT-induced melanogenesis and searching the mechanism. In our study, IFN-gamma attenuated 5-HT-induced pigmentation and green fluorescent protein (GFP) expression in zebrafishes. In addition, we found that IFN-gamma decreased serum serotonin levels as well as the cutaneous expression of tryptophan hydroxylase 1 (TPH1), 5-HT1A receptor (5-HT1AR) and 5-HT1B receptor (5-HT1BR) in C57BL/6 mice. Moreover, IFN-gamma attenuated 5-HT-induced melanin biosynthesis as well as the expression of 5-HT1AR, 5-HT1BR and 5-HT2A receptor (5HT2AR) in 616F10 cells, which blocked by interferon-gamma receptor 1 and interferon-gamma receptor 2 (IFNGR1/IFNGR2) antibodies. In summary, IFN-gamma not only affects melanogenesis alone, but also inhibits 5-HT response on melanin biosynthesis. Mediated by IFNGR1/IFNGR2, IFN-gamma downregulated 5-HT receptors expression, which directly affect 5-HT-induced melanin biosynthesis. Our work may give insights into the drug development of hypopigmentation disorders with neuro-immune derangement. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.
关键词	IFN-gamma 5-HT Melanin biosynthesis 5-HT receptors
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59710
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Cai, Minxuan,Zhou, Liangliang,Liao, Jun,et al. IFN-gamma inhibits 5-HT-induced melanin biosynthesis via downregulation of 5-HT receptors in vivo/in vitro[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2019,141(1).
APA	Cai, Minxuan,Zhou, Liangliang,Liao, Jun,Huang, Qiaoling,Xia, Zhenjiang,&Shang, Jing.(2019).IFN-gamma inhibits 5-HT-induced melanin biosynthesis via downregulation of 5-HT receptors in vivo/in vitro.JOURNAL OF PHARMACOLOGICAL SCIENCES,141(1).
MLA	Cai, Minxuan,et al."IFN-gamma inhibits 5-HT-induced melanin biosynthesis via downregulation of 5-HT receptors in vivo/in vitro".JOURNAL OF PHARMACOLOGICAL SCIENCES 141.1(2019).