Studies of hTERT DNA methylation assays on the human age prediction

NWIPB OpenIR

	Studies of hTERT DNA methylation assays on the human age prediction
	Xin, Ye; Dong, Kaikai; Cao, Fangqi; Tian, Yuxiang; Sun, Jing; Peng, Min; Liu, Wenbin; Shi, Ping
	2019
发表期刊	INTERNATIONAL JOURNAL OF LEGAL MEDICINE
卷号	133 期号:5
摘要	As an important aspect of epigenetics, DNA methylation has been proven to be suitable for forensic DNA analysis. By detecting changes in DNA methylation, it is desirable to construct a model of age patterns associated with it to infer the age of the individual. The hTERT gene methylation is closely related to tumors, but there are few reports on the relationship between hTERT gene promoter methylation and age. In this study, we utilized the methylation-specific polymerase chain reaction and real-time PCR (relative quantification and absolute quantification) approach to explore the connection between hTERT DNA methylation and age prediction. We fit three models for age prediction based on methylation assay for 90 blood samples from donors aged 1-79 years old. Among them, the model of absolute quantification of real-time enabled the age prediction with R-2 = 0.9634. We verified the linear regression model with a validation set of 30 blood samples where prediction average error was 4.29 years. Generally, this reliable method improves the DNA methylation analysis of forensic samples.
关键词	DNA methylation Age prediction MSP Real-time PCR
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59714
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xin, Ye,Dong, Kaikai,Cao, Fangqi,et al. Studies of hTERT DNA methylation assays on the human age prediction[J]. INTERNATIONAL JOURNAL OF LEGAL MEDICINE,2019,133(5).
APA	Xin, Ye.,Dong, Kaikai.,Cao, Fangqi.,Tian, Yuxiang.,Sun, Jing.,...&Shi, Ping.(2019).Studies of hTERT DNA methylation assays on the human age prediction.INTERNATIONAL JOURNAL OF LEGAL MEDICINE,133(5).
MLA	Xin, Ye,et al."Studies of hTERT DNA methylation assays on the human age prediction".INTERNATIONAL JOURNAL OF LEGAL MEDICINE 133.5(2019).