Highly selective and sensitive peptide-based fluorescent chemosensor for detection of Zinc(II) ions in aqueous medium and living cells

NWIPB OpenIR

	Highly selective and sensitive peptide-based fluorescent chemosensor for detection of Zinc(II) ions in aqueous medium and living cells
	Wang, Peng; Wu, Xuehua; Wu, Jiang; Liao, Yunwen
	2019
发表期刊	JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
卷号	382
摘要	A novel peptide-based chemosensor (L, Dansyl-Asp-His-NH2) has been successfully designed and synthesized. L displayed excellent selectivity in fluorescence detection of Zn2+ over other essential metal ions, with a binding constant value of 4.09 x 10(10) M-2. Further, a Job's plot confirmed the 2:1 stoichiometry of the complex formation between the L and Zn2+ ions. L exhibited a highly-sensitive strong fluorescence "turn-on" effect for Zn2+ in aqueous solution, with a detection limit for Zn2+ calculated at 36.8 nM. Notably, L served as a recyclable chemosensor, which was cyclically controlled by Zn2+ and Na(2)EDTA. Moreover, L exhibited low toxicity in HK2 normal cells, and was then applied to monitor intracellular Zn2+ and Na(2)EDTA in human prostate cancer cells (LNCaP cells).
关键词	Peptide Chemosensor Reversibility Zn(II) ions Cells imaging
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59716
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Peng,Wu, Xuehua,Wu, Jiang,et al. Highly selective and sensitive peptide-based fluorescent chemosensor for detection of Zinc(II) ions in aqueous medium and living cells[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,2019,382.
APA	Wang, Peng,Wu, Xuehua,Wu, Jiang,&Liao, Yunwen.(2019).Highly selective and sensitive peptide-based fluorescent chemosensor for detection of Zinc(II) ions in aqueous medium and living cells.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,382.
MLA	Wang, Peng,et al."Highly selective and sensitive peptide-based fluorescent chemosensor for detection of Zinc(II) ions in aqueous medium and living cells".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY 382(2019).