A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect

NWIPB OpenIR

	A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect
	Zhu, Shuyun; Yan, Xiaolu; Sun, Jing; Zhao, Xian-En; Wang, Xiao
	2019
发表期刊	TALANTA
卷号	200
摘要	The authors describe a novel method for the determination of artemisinin (ART) by using graphene quantum dots (GQDs) as the fluorescent probes. This method is based on the fact that ART can react with p-aminophenylboronic acid (p-ABA) to produce p-aminophenol (p-AP). While in the presence of tyrosinase (TYR), p-AP can be oxidized into 4-amino-1,2-benzoquinone, which effectively quenched the fluorescence of GQDs due to the inner filter effect (IFE). By making use of these reactions, a novel and sensitive fluorescent assay for ART has been developed. The calibration curve for the determination of ART is linear in the range of 0.1-5 mu M and 5-55 mu M with the detection limit of 33 nM, which is more sensitive than most of other methods. Some common coexisting substances including Ca2+, Na+, Mg2+, K+, PO43-, starch, lactose, dextrin, and magnesium stearat have negligible effects on the fluorescence intensity of GQDs-TYR-p-ABA system. Finally, the sensing system was successfully applied to the detection of the compound naphthoquine phosphate tablet samples with satisfactory recoveries. This IFE-based GQDs fluorescence sensing strategy is facile and sensitive for the determination of ART because neither the surface modification nor the linking between the receptor and the fluorophore is required.
关键词	Artemisinin Graphene quantum dots Fluorescent assay Inner filter effect Pharmaceutical analysis
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59739
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhu, Shuyun,Yan, Xiaolu,Sun, Jing,et al. A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect[J]. TALANTA,2019,200.
APA	Zhu, Shuyun,Yan, Xiaolu,Sun, Jing,Zhao, Xian-En,&Wang, Xiao.(2019).A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect.TALANTA,200.
MLA	Zhu, Shuyun,et al."A novel and sensitive fluorescent assay for artemisinin with graphene quantum dots based on inner filter effect".TALANTA 200(2019).