Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak

NWIPB OpenIR

	Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak
	Wang, Guowen; Li, Yongchang; Yang, Qilin; Xu, Shangrong; Ma, Shike; Yan, Rongge; Zhang, Ruina; Jia, Gongxue; Ai, Deqiang; Yang, Qi'en
	2019
发表期刊	JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
卷号	10
摘要	BackgroundSpermatogenesis is a cellular differentiation process that includes three major events: mitosis of spermatogonia, meiosis of spermatocytes and spermiogenesis. Steady-state spermatogenesis relies on functions of spermatogonial stem cells (SSCs). Establishing and maintaining a foundational SSC pool is essential for continued spermatogenesis in mammals. Currently, our knowledge about SSC and spermatogenesis is severely limited in domestic animals.ResultsIn the present study, we examined transcriptomes of testes from domestic yaks at four different stages (3, 5, 8 and 24months of age) and attempted to identify genes that are associated with key developmental events of spermatogenesis. Histological analyses showed that the most advanced germ cells within seminiferous tubules of testes from 3, 5, 8 and 24months old yaks were gonocytes, spermatogonia, spermatocytes and elongated spermatids, respectively. RNA-sequencing (RNA-seq) analyses revealed that 11904, 4381 and 2459 genes were differentially expressed during the gonocyte to spermatogonia transition, the mitosis to meiosis transition and the meiosis to post-meiosis transition. Further analyses identified a list of candidate genes than may regulate these important cellular processes. CXCR4, a previously identified SSC niche factor in mouse, was one of the up-regulated genes in the 5months old yak testis. Results of immunohistochemical staining confirmed that CXCR4 was exclusively expressed in gonocytes and a subpopulation of spermatogonia in the yak testis.ConclusionsTogether, these findings demonstrated histological changes of postnatal testis development in the domestic yak. During development of spermatogonial lineage, meiotic and haploid germ cells are supported by dynamic transcriptional regulation of gene expression. Our transcriptomic analyses provided a list of candidate genes that potentially play crucial roles in directing the establishment of SSC and spermatogenesis in yak.
关键词	Gonocyte Meiosis Spermatogenesis Spermatogonia Transcriptome
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59745
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Guowen,Li, Yongchang,Yang, Qilin,et al. Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak[J]. JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY,2019,10.
APA	Wang, Guowen.,Li, Yongchang.,Yang, Qilin.,Xu, Shangrong.,Ma, Shike.,...&Yang, Qi'en.(2019).Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak.JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY,10.
MLA	Wang, Guowen,et al."Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak".JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY 10(2019).