Development of EST-SSR markers in Saxifraga sinomontana (Saxifragaceae) and cross-amplification in three related species

NWIPB OpenIR

	Development of EST-SSR markers in Saxifraga sinomontana (Saxifragaceae) and cross-amplification in three related species
	Li, Yan; Jia, Liu-Kun; Zhang, Fa-Qi; Wang, Zhi-Hua; Chen, Shi-Long; Gao, Qing-Bo
	2019
发表期刊	APPLICATIONS IN PLANT SCIENCES
卷号	7 期号:6
摘要	Premise Saxifraga sinomontana (Saxifragaceae) is a widespread alpine species in the Qinghai-Tibetan Plateau and its flanking mountains. We developed a set of expressed sequence tag-simple sequence repeat (EST-SSR) markers to investigate the genetic diversity and evolutionary history of the species. Methods and Results We initially designed 50 EST-SSR markers based on transcriptome data of S. sinomontana. Nineteen of 50 loci (38%) were successfully amplified, 13 of which were polymorphic. These were tested on 71 individuals from four populations. Three to 18 alleles per locus were detected, and the levels of observed and expected heterozygosity ranged from 0.2817 to 0.9155 and 0.2585 to 0.8495, respectively. In addition, cross-amplification was successful for all 13 loci in three congeneric species, S. tangutica, S. heleonastes, and S. congestiflora. Conclusions These EST-SSR markers will be useful for studying the genetic diversity of S. sinomontana and disentangling the phylogenetic relationships of related species.
关键词	EST-SSR markers Saxifraga congestiflora Saxifraga heleonastes Saxifraga sinomontana Saxifraga tangutica transcriptome
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59778
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Yan,Jia, Liu-Kun,Zhang, Fa-Qi,et al. Development of EST-SSR markers in Saxifraga sinomontana (Saxifragaceae) and cross-amplification in three related species[J]. APPLICATIONS IN PLANT SCIENCES,2019,7(6).
APA	Li, Yan,Jia, Liu-Kun,Zhang, Fa-Qi,Wang, Zhi-Hua,Chen, Shi-Long,&Gao, Qing-Bo.(2019).Development of EST-SSR markers in Saxifraga sinomontana (Saxifragaceae) and cross-amplification in three related species.APPLICATIONS IN PLANT SCIENCES,7(6).
MLA	Li, Yan,et al."Development of EST-SSR markers in Saxifraga sinomontana (Saxifragaceae) and cross-amplification in three related species".APPLICATIONS IN PLANT SCIENCES 7.6(2019).