A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography

NWIPB OpenIR

	A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography
	Wang, Nana; Chen, Tao; Yang, Xue; Shen, Cheng; Li, Hongmei; Wang, Shuo; Zhao, Jingyang; Chen, Jilin; Chen, Zhi; Li, Yulin
	2019
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	42 期号:9
摘要	Separation of minor compounds especially with similar polarities and structures from complex samples is a challenging work. In the present study, an efficient method was successfully established by macroporous resin column chromatography, medium-pressure liquid chromatography, and high-speed countercurrent chromatography for separation of four minor flavonoids from barley seedlings. Macroporous resin column chromatography and medium-pressure liquid chromatography were used for enrichment of these four flavonoids. High-pressure liquid chromatography analysis showed the total content of these four flavonoids increased from 2.2% in the crude extract to 95.3% in the medium-pressure liquid chromatography fraction. It was indicated that the combination of macroporous resin column chromatography and medium-pressure liquid chromatography could be a practicable strategy for enrichment of minor compounds from complex sample. Then, high-speed countercurrent chromatography was employed for separation of these four flavonoids using ethyl acetate/n-butanol/water (0.1% glacial acetic acid) (4: 1: 5, v/v/v) as solvent system. As a result, four flavonoids including two isomers with purities higher than 98% were obtained. Interestingly, two flavonoids existing in one high-pressure liquid chromatography peak were also successfully separated. All these indicated high-speed countercurrent chromatography had great potential for separation of compounds with similar structures and polarities. This study provides a reference for efficient enrichment and separation of minor compounds from complex sample.
关键词	barley seedlings high-speed counter-current chromatography isomers minor flavonoids
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59796
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Nana,Chen, Tao,Yang, Xue,et al. A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(9).
APA	Wang, Nana.,Chen, Tao.,Yang, Xue.,Shen, Cheng.,Li, Hongmei.,...&Li, Yulin.(2019).A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography.JOURNAL OF SEPARATION SCIENCE,42(9).
MLA	Wang, Nana,et al."A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium-pressure LC, and high-speed countercurrent chromatography".JOURNAL OF SEPARATION SCIENCE 42.9(2019).