Bacterial community in cold and alkaline environments of Hoh Xil basin in Qinghai-Tibet Plateau and isolation of potential sources of microbiota

NWIPB OpenIR

	Bacterial community in cold and alkaline environments of Hoh Xil basin in Qinghai-Tibet Plateau and isolation of potential sources of microbiota
	Xing, Rui; Gao, Qing-bo; Zhang, Fa-qi; Wang, Jiu-li; Chen, Shi-long
	2019
发表期刊	ANNALS OF MICROBIOLOGY
卷号	69 期号:5
摘要	The Hoh Xil basin is the largest Cenozoic sedimentary basin in the Qinghai-Tibet Plateau (QTP) with an average altitude of above 5000m. It is also the coldest region in the QTP. However, due to the difficulty of sample collection caused by the harsh natural environment, a limited number of studies have been conducted on soil microorganisms in this region. We used culture-dependent and independent methods to investigate the bacterial communities in desert soil (n1), saline-alkali land (n2), saline-alkali wetland (n3), and soda lake sediment (n4). The results showed distinct bacterial communities between different environmental types. We found that the Chao1 and Shannon diversity indices of n1 were significantly lower than those of n4 (P<0.05). At the phylum level, all samples were dominated by representatives of Proteobacteria, Bacteroidetes, and Actinobacteria, which were similar to the findings of previous studies on the desert soil in the same region. Moreover, we identified 10 strains of bacteria from 109 isolates, most of which belonged to Pseudomonas (90.8%). The growth of the isolate k9 was optimal at a high pH value (pH10.0) and a low temperature (5 degrees C), and this stain could produce extracellular enzyme (alkaline phosphatase, acid phosphatase, and naphthol-AS-BI-phosphohydrolase) under alkaline (pH10) and cold (5 degrees C) condition. These results demonstrate the diversity of bacteria in the Hoh Xil basin and identify potential psychrophilic and alkaliphilic bacteria with multiple types of extracellular enzyme activity.
关键词	Qinghai-Tibet Plateau Alkaliphiles bacteria Psychrophilic bacteria Psychrotolerant alkaliphile Microbial community
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59803
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xing, Rui,Gao, Qing-bo,Zhang, Fa-qi,et al. Bacterial community in cold and alkaline environments of Hoh Xil basin in Qinghai-Tibet Plateau and isolation of potential sources of microbiota[J]. ANNALS OF MICROBIOLOGY,2019,69(5).
APA	Xing, Rui,Gao, Qing-bo,Zhang, Fa-qi,Wang, Jiu-li,&Chen, Shi-long.(2019).Bacterial community in cold and alkaline environments of Hoh Xil basin in Qinghai-Tibet Plateau and isolation of potential sources of microbiota.ANNALS OF MICROBIOLOGY,69(5).
MLA	Xing, Rui,et al."Bacterial community in cold and alkaline environments of Hoh Xil basin in Qinghai-Tibet Plateau and isolation of potential sources of microbiota".ANNALS OF MICROBIOLOGY 69.5(2019).