A highly sensitive near-infrared ratiometric fluorescent probe for imaging of mitochondrial hydrazine in cells and in mice models

NWIPB OpenIR

	A highly sensitive near-infrared ratiometric fluorescent probe for imaging of mitochondrial hydrazine in cells and in mice models
	Song, Yaqun; Chen, Guang; Han, Xiaoyue; You, Jinmao; Yu, Fabiao
	2019
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	286
摘要	Hydrazine (N2H4) has been listed as a possible human carcinogen. It can cause severe damage to various organs through respiratory tract, skin and digestive tract. Although some fluorescent probes have been developed for imaging of N2H4, there is very few entry of application in vivo, probably limited by the short fluorescence emission wavelengths. Moreover, the sensitivity also needs to be improved to meet the requirement of detection in vivo. Hence, we design and synthesize a new NIR ratiometric fluorescent probe Cy-OAc based on cyanine derivatives for the sensitive detection of N2H4 in cells and in mice models. Cy-OAc consists of a large pi-conjugated system and a masking unit for both regulating the pi-system and tracing N2H4. Cy-OAc holds the emission profile centered at 825 nm under the excitation of 730 nm, and upon reaction with N2H4, it shifts the emission profile centered at 662 nm with an excitation at 540 nm, resulting in the NIR ratiometric emission response, as well as a distinct color change that can be distinguished by the naked eye. Cy-OAc has been successfully applied to imaging the N2H4 in apoptotic cells. The results reveal that N2H4 can lead to the apoptosis of HeLa cells via the damage of mitochondrial membrane potential. Taking advantage of the NIR emissions, Cy-OAc exhibits the deep tissue penetration of 700 mu m in kidney tissue. Our probe Cy-OAc is further utilized to visually detect N(2)H(4-)metabolism and estimate kidney damage in mice models. We envision that the probe Cy-OAc can be an attractive chemical tool for exploring N2H4 in bio-specimen.
关键词	Fluorescent probe Near-infrared fluorescence Mitochondrial hydrazine Imaging analysis
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59810
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Song, Yaqun,Chen, Guang,Han, Xiaoyue,et al. A highly sensitive near-infrared ratiometric fluorescent probe for imaging of mitochondrial hydrazine in cells and in mice models[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,286.
APA	Song, Yaqun,Chen, Guang,Han, Xiaoyue,You, Jinmao,&Yu, Fabiao.(2019).A highly sensitive near-infrared ratiometric fluorescent probe for imaging of mitochondrial hydrazine in cells and in mice models.SENSORS AND ACTUATORS B-CHEMICAL,286.
MLA	Song, Yaqun,et al."A highly sensitive near-infrared ratiometric fluorescent probe for imaging of mitochondrial hydrazine in cells and in mice models".SENSORS AND ACTUATORS B-CHEMICAL 286(2019).