Evaluation of hepatotoxicity potential of a potent traditional Tibetan medicine Zuotai

NWIPB OpenIR

	Evaluation of hepatotoxicity potential of a potent traditional Tibetan medicine Zuotai
	Zhou, Liang-Liang; Chen, Hai-Juan; He, Qiang-Qiang; Li, Cen; Wei, Li-Xin; Shang, Jing
	2019
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
卷号	234
摘要	Ethnopharmacological relevance: Zuotai (gTso thal) has a long history in the treatment of cardiovascular disease, liver and bile diseases, spleen and stomach diseases as a precious adjuvant in Tibetan medicine. However, Zuotai is a mercury preparation that contains 54.5% HgS. Its application has always been controversial. Aim of the study: To evaluate the toxicological effects of Zuotai in hepatocytes and in zebrafish. Materials and methods: MTT was used to determine the survival rate of hepatocytes; Hoechst and TUNEL staining were used to detect the apoptosis cells; Western blot and RT-qPCR assay were used to determine the expression levels of the protein and mRNA; Liver morphology observation and H&E staining were used to evaluate the hepatotoxicity of Zuotai in Zebfrafish. Results: The survival rate of L-02 cells, HepG2 cells and RBL-2A cells reduced by Zuotai (10(-4)-0.1 mg/mL) in a dose and time-dependent manner. Zuotai (0.1 mg/mL) induced HepG2 cells shrinkage, condensation and fragmentation and increased the number of apoptosis cells. The protein expression levels of cleaved Caspase-3 and Bax were increased and the expression levels of Bcl-2 were reduced after HepG2 cells exposed to Zuotai (10(-4)-0.1 mg/mL) for 24 h. In addition, Zuotai (0.2 mg/mL) induced the darker liver color of the larval zebrafish and changed the liver morphologic of adult zebrafish. Zuotai (0.2 mg/mL) also increased the mRNA levels of CYP1A1, CYP1B1 and MT-1 in the liver of adult zebrafish. However, no significantly hepatotoxicity was observed after hepatocytes and zebrafish exposed to HgS at the same dose. Conclusions: Results showed that Zuotai induced hepatotoxicity effectively under a certain dose but its hepatotoxicity likely occurs via other mechanisms that did not depend on HgS.
关键词	Zuotai HgS Hepatotoxicity Hepatocytes Zebrafish
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/59813
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhou, Liang-Liang,Chen, Hai-Juan,He, Qiang-Qiang,et al. Evaluation of hepatotoxicity potential of a potent traditional Tibetan medicine Zuotai[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,234.
APA	Zhou, Liang-Liang,Chen, Hai-Juan,He, Qiang-Qiang,Li, Cen,Wei, Li-Xin,&Shang, Jing.(2019).Evaluation of hepatotoxicity potential of a potent traditional Tibetan medicine Zuotai.JOURNAL OF ETHNOPHARMACOLOGY,234.
MLA	Zhou, Liang-Liang,et al."Evaluation of hepatotoxicity potential of a potent traditional Tibetan medicine Zuotai".JOURNAL OF ETHNOPHARMACOLOGY 234(2019).